Validation of an automated computational method for skeletal muscle fibre morphometry analysis

Neuromuscular Disorders : NMD
Fleur GartonNan Yang

Abstract

Accurate and fast measurement of muscle fibre size and evaluation of fibre type proportions in large cross-sectional areas remains challenging as existing methods require extensive manual measurements. In this study, we assessed the fibre morphometry of approximately 1000 fibres in mouse and human control and diseased muscle cross-sections. We compared fibre size, percentage fibre proportion and percentage fibre surface area results obtained by an automated method using MetaMorph with those obtained manually using Image Pro. Data collection using MetaMorph software was faster and produced similar results to those obtained using Image Pro. The ability to quickly and accurately measure large numbers of fibres with MetaMorph allows the researcher to make a more precise assessment of fibre type and fibre size changes in human muscle biopsies and animal models of muscle disease.

References

Jun 29, 2000·Biochemical and Biophysical Research Communications·C A LucasJ F Hoh
Sep 17, 2004·Muscle & Nerve·Visalini Nair-ShallikerEdna C Hardeman
Oct 7, 2006·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Thomas Brox, Joachim Weickert

❮ Previous
Next ❯

Citations

Dec 24, 2011·Acta Physiologica·S LarsenF Dela
Oct 5, 2013·The Journal of Clinical Investigation·Jane T SetoKathryn N North
Oct 29, 2013·Human Molecular Genetics·Kate SullivanAaron Schindeler
Nov 30, 2010·Experimental Gerontology·Jane T SetoKathryn N North
Feb 17, 2015·Journal of the South African Veterinary Association·Maurice Mars, Michael A Gregory
Feb 1, 2017·Nature Communications·Marshall W HogarthKathryn N North
Jun 22, 2019·Journal of Cachexia, Sarcopenia and Muscle·Christian M GirgisJenny E Gunton
Aug 18, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Emily J GreenspanFung-Lung Chung
Apr 30, 2017·Nature Communications·Esther LatresJesper Gromada
Dec 12, 2017·Human Molecular Genetics·Matthew A SummersAaron Schindeler
May 1, 2018·American Journal of Human Genetics·Fleur C GartonKathryn N North

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.