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Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

Nucleic Acids Research

Feb 2, 2013

James ChappellPaul S Freemont

Abstract

A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which...read more

Mentioned in this Paper

Alkalescens-Dispar Group
Transcription, Genetic
Protein Biosynthesis
Gene Amplification Technique
Genes, Bacterial
Sequence Determinations, DNA
T4 DNA Ligase
DNA, Circular
cDNA Library
Gene Amplification Abnormality
20
2
7
245
Paper Details
References
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  • Citations37
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Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

Nucleic Acids Research

Feb 2, 2013

James ChappellPaul S Freemont

PMID: 23371936

DOI: 10.1093/nar/gkt052

Abstract

A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which...read more

Mentioned in this Paper

Alkalescens-Dispar Group
Transcription, Genetic
Protein Biosynthesis
Gene Amplification Technique
Genes, Bacterial
Sequence Determinations, DNA
T4 DNA Ligase
DNA, Circular
cDNA Library
Gene Amplification Abnormality
20
2
7
245

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Proceedings of the National Academy of Sciences of the United States of America

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways

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Laura M Wingler, Virginia W Cornish
ACS Synthetic Biology

Ensemble Bayesian analysis of bistability in a synthetic transcriptional switch

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Paper Details
References
  • References52
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12345...
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1234
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