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Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

Nucleic Acids Research

Mar 1, 2013

James ChappellPaul A Freemont

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Abstract

A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which...read more

Mentioned in this Paper

Protein Biosynthesis
DNA, Bacterial
Early Promoters, Genetic
Sequence Determinations, DNA
Gene Activation
Cell-Free System
cDNA Library
T4 DNA Ligase
Regulatory Sequences, Nucleic Acid
In Vitro [Publication Type]
20
2
7
241
Paper Details
References
  • References52
  • Citations36
1234

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

Nucleic Acids Research

Mar 1, 2013

James ChappellPaul A Freemont

PMID: 23371936

DOI: 10.1093/nar/gkt052

Abstract

A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which...read more

Mentioned in this Paper

Protein Biosynthesis
DNA, Bacterial
Early Promoters, Genetic
Sequence Determinations, DNA
Gene Activation
20
2
7
241

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Paper Details
References
  • References52
  • Citations36
1234

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