PMID: 8939396Dec 1, 1996Paper

Validation of candidate reference methods based on ion chromatography for determination of total sodium, potassium, calcium and magnesium in serum through comparison with flame atomic emission and absorption spectrometry

Clinical Biochemistry
L M ThienpontD Stöckl

Abstract

We report on the validation of candidate reference methods based on ion chromatography for the determination of total sodium, potassium, calcium, and magnesium in serum. The current study is a continuation of previous investigations in which we were able to show the potential of ion chromatography to serve as a reference method principle. The validation consisted of comparison of the ion chromatographic methods with generally recognized reference methods based on flame atomic emission (for sodium/ potassium) and absorption (for calcium/magnesium) spectrometry. Sample preparation consisted of acidic dilution and pre-purification with reversed phase cartridges. Ion chromatography with suppressed conductivity was performed with polymeric stationary phases chemically modified with carboxylate and sulfonate functional groups for sodium/potassium and calcium/magnesium, respectively. Single-point calibration was used in combination with individual adaptation of serum sampling and diluting to give similar responses in samples and standards. In the study, more than 25 control sera were analyzed in parallel with the ion chromatographic methods and the traditional reference methods. The measurement protocol consisted of quadruplicate anal...Continue Reading

References

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Citations

Feb 25, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·K DewitteL M Thienpont
May 16, 1997·Journal of Chromatography. a·J E Van NuwenborgL M Thienpont
Apr 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·J E Van NuwenborgL M Thienpont
Jul 17, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jean-Jacques LahetBernard Chaillot
Apr 14, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Eric D B GouletLindsay B Baker
Apr 1, 2018·Journal of Clinical Laboratory Analysis·Jihua ZouBingde Zou
Apr 1, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Ying YanChuanbao Zhang
Oct 15, 2019·Temperature : Multidisciplinary Biomedical Journal·Lindsay B Baker
Dec 13, 2016·Clinical Biochemistry·Peter A Kavsak
Jun 17, 1999·Annals of Clinical Biochemistry·J E Van NuwenborgJ Kratochvíla
Jun 29, 1999·Analytical Chemistry·D J Anderson

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