Validation of cultivation and PCR methods for diagnosis of Lyme neuroborreliosis.

Journal of Clinical Microbiology
Tjaša CerarEva Ružič-Sabljič

Abstract

Borrelial infection may manifest with a wide range of clinical signs, and in many cases, microbiological findings are essential for a proper diagnosis. This study included 48 patients with a working clinical diagnosis of Lyme neuroborreliosis, 45 patients with a working clinical diagnosis of suspected Lyme neuroborreliosis, and a control group comprising 42 patients with tick-borne encephalitis and 21 neurosurgical patients. The aim of the study was to analyze and compare findings of two PCR methods and Borrelia burgdorferi sensu lato culture results by examination of prospectively collected cerebrospinal fluid (CSF) and blood specimens from patients with clinical features of Lyme neuroborreliosis. Borrelial DNA was detected with at least one of the PCR approaches in 16/135 (11.9%) blood samples and 24/156 (15.4%) CSF samples. Using MseI restriction of PCR products of the amplified rrf-rrl region, we identified the majority of strains as Borrelia afzelii. Borreliae were isolated from 1/135 (0.7%) blood samples and from 5/156 (3.2%) CSF specimens. Using MluI restriction for characterization of isolated strains, Borrelia garinii was identified in all CSF isolates. Our study revealed that different approaches for direct demonstrat...Continue Reading

References

Feb 1, 1991·American Journal of Clinical Pathology·C E GreerM M Manos
Apr 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R N PickenG M Trenholme
Jul 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F StrleR N Picken
Jan 1, 1997·Clinical Microbiology Reviews·B L Schmidt
Sep 14, 1999·Medical and Veterinary Entomology·E I KorenbergG G Moskvitina
Feb 1, 2000·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F Strle
Jul 14, 2001·The New England Journal of Medicine·A C Steere
May 2, 2002·The Medical Clinics of North America·P K Coyle, S E Schutzer
Nov 25, 2003·Lancet·Gerold Stanek, Franc Strle
Dec 21, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P BrouquiUNKNOWN European Network for Surveillance of Tick-Borne Diseases
Jul 16, 2005·Clinical Microbiology Reviews·Maria E Aguero-RosenfeldGary P Wormser
Aug 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F StrleV Maraspin
Feb 3, 2007·FEMS Immunology and Medical Microbiology·Bettina WilskeUlrike Schulte-Spechtel

❮ Previous
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Citations

Nov 26, 2009·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A MyglandUNKNOWN European Federation of Neurological Societies
Nov 23, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A J HenningssonA Matussek
Apr 18, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A BrandaAllen C Steere
May 15, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gary P Wormser, John J Halperin
Sep 29, 2015·Expert Review of Vaccines·Renata Šmit, Maarten J Postma
Mar 1, 2011·Expert Opinion on Medical Diagnostics·Alje P van Dam
Dec 1, 2009·Neurobiology of Disease·Brian A FallonDavid Hardesty
Aug 26, 2011·Journal of Neuropathology and Experimental Neurology·Simone C TauberRoland Nau
Dec 5, 2012·Acta Neurologica Scandinavica. Supplementum·U Ljøstad, Å Mygland
Nov 26, 2015·Clinics in Laboratory Medicine·John J Halperin
Nov 26, 2015·Clinics in Laboratory Medicine·Kevin Alby, Gerald A Capraro
Aug 9, 2015·BMC Veterinary Research·Tjaša CerarEva Ružić-Sabljić
Jul 29, 2015·Nature Reviews. Neurology·Uwe KoedelHans-Walter Pfister
Nov 19, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E Ružić-SabljićT Cerar
May 23, 2015·Infectious Disease Clinics of North America·Adriana R Marques
Dec 3, 2014·Journal of Autoimmunity·Andrea T BorchersM Eric Gershwin
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Stanek, M Reiter
Oct 8, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L MaesM Raymaekers
Nov 29, 2016·Expert Review of Molecular Diagnostics·Eva Ružić-Sabljić, Tjaša Cerar
Aug 16, 2013·Clinical and Vaccine Immunology : CVI·T CerarE Ruzić-Sabljic
Sep 28, 2018·Journal of Clinical Microbiology·Elitza S TheelGary P Wormser
Jul 3, 2009·Current Opinion in Infectious Diseases·Gerold Stanek, Franc Strle
Oct 31, 2019·Clinical and Experimental Immunology·T van GorkomS F T Thijsen
Feb 19, 2010·Clinical and Vaccine Immunology : CVI·Tjasa CerarEva Ruzić-Sabljić
Jun 2, 2020·Expert Review of Anti-infective Therapy·Ilias MasourisUwe Ködel
May 28, 2020·BMJ : British Medical Journal·Bart Jan KullbergJoppe W Hovius
Feb 11, 2011·Anais Brasileiros De Dermatologia·Mônica SantosSinésio Talhari
Feb 20, 2020·MMW Fortschritte der Medizin·Uwe KödelHans-Walter Pfister
Jun 10, 2009·International Journal of Immunopathology and Pharmacology·I SantinoM Del Piano
Jun 13, 2018·FEMS Microbiology Reviews·Gerold Stanek, Franc Strle
Jan 3, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Barbro H SkogmanPer-Eric Lindgren
Nov 4, 2020·Biology·Victoria P SandersonMelanie K B Wills
Jan 29, 2021·Clinical Microbiology Reviews·John A Branda, Allen C Steere
Jul 2, 2019·Médecine et maladies infectieuses·X GockoUNKNOWN endorsed by the following scientific societies

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