Validation of HPLC-UV methods for quantitatively determining landiolol and its major metabolite in human blood

Biological & Pharmaceutical Bulletin
Yoshitaka HashimotoYasuyuki Miyata

Abstract

Landiolol is an ultra-short-acting beta(1)-adrenergic receptor blocking agent that is used for both perioperative and postoperative patients with tachycardia during general anesthesia. Validated HPLC-UV methods that quantitatively determine landiolol and its major metabolite (M-1) in human blood were reported for clinical research of landiolol. These analytes were recovered from the same blood sample using a multi-step extraction process and determined with two different HPLC conditions. These methods were validated over concentration ranges of 0.05 to 10 microg/ml for landiolol and 0.1 to 20 microg/ml for M-1 and were found to have acceptable accuracy, precision, linearity, and selectivity. These methods are useful to the characterize of blood pharmacokinetics of landiolol and M-1 for clinical research.

Citations

Nov 19, 2010·Bioanalysis·Pranav S ShrivastavMallika Sanyal

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