Validation of Multi-Residue Method for Quantification of Antibiotics and NSAIDs in Avian Scavengers by Using Small Amounts of Plasma in HPLC-MS-TOF

International Journal of Environmental Research and Public Health
Pilar Gómez-RamírezAntonio Juan García-Fernández

Abstract

Pharmaceuticals are still considered emerging pollutants affecting both aquatic and terrestrial ecosystems. Scavenging bird species may be exposed to veterinary drugs when they feed on livestock carcasses provided at supplementary feeding stations, as these are often stocked with ailing and/or recently medicated animals. Because those animals may be a source of several different pharmaceutical compounds, analytical methods to evaluate residue levels and exposure potential should enable detection and quantification of as many different compounds as possible, preferably from small sample volumes. Four different extraction methods were tested to conduct HPLC-MS-TOF analysis of some of the most common veterinary drugs used in livestock in Spain. The method deemed most viable was a simple extraction, using methanol and 100 µL of plasma, that allowed quantification of seven antibiotics (tetracycline, oxytetracycline, ciprofloxacin, enrofloxacin, nalidixic acid, trimethoprim, sulfadiazine) and five nonsteroidal anti-inflammatory drugs (NSAIDs) (meloxicam, flunixin, carprofen, tolfenamic acid, phenylbutazone). The method was then applied to analysis of 29 Eurasian griffon vulture (Gyps fulvus) nestling samples, wherein enrofloxacin and...Continue Reading

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May 14, 2020·Ambio·Silvia EspínAntonio J García-Fernández

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Citations

Apr 4, 2021·International Journal of Environmental Research and Public Health·Nayden Chakarov, Guillermo Blanco
Jan 13, 2022·The Science of the Total Environment·Pablo I PlazaSergio A Lambertucci

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Agilent Mass Hunter Data Acquisition
QuEChERS
Excel
Agilent Mass Hunter Qualitative Analysis

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