Validation of PCR-reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
A M C BergmansR G F Wintermans

Abstract

A dermatophyte-specific PCR-reverse line blot (PCR-RLB) assay based on internal transcribed sequences was developed. This assay allows the rapid detection and identification of nine clinically relevant species within the three dermatophyte genera Trichophyton, Microsporum and Epidermophyton in nail, skin and hair samples within 1 day. Analysis of 819 clinical samples (596 nail, 203 skin and 20 hair) revealed a positive PCR-RLB result in 93.6% of 172 culture-positive and microscopy-positive samples. PCR-RLB was superior to culture and direct microscopy, in both detection and species identification. Comparison of identification results of 208 PCR-positive and culture-positive clinical samples showed five discrepancies (2.4%) between PCR-RLB identification and classical microscopic/biochemical identification of isolates. Comparison of PCR-RLB identification and classical identification of 98 other isolates (dermatophytes and non-dermatophytes) revealed 13 discrepancies (13.3%) and five incomplete identifications of Trichophyton spp. Sequence analysis of ITS1 regions of 23 samples with discrepant or incomplete identification results (four Centraalbureau voor Schimmelcultures dermatophyte strains, four clinical samples and 15 clinic...Continue Reading

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Citations

Dec 24, 2010·Journal of Clinical Microbiology·Qiaoyun LuG S de Hoog
Apr 21, 2009·BMC Research Notes·Jaya GargGopal Nath
Dec 3, 2014·Journal de mycologie médicale·D Chabasse, M Pihet
Feb 13, 2016·Medical Mycology·Bahram AhmadiNiloofar Jalalizand
Dec 1, 2008·Annales de dermatologie et de vénéréologie·P Berbis
Jul 2, 2014·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Pietro NenoffYvonne Gräser
Feb 14, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A BergmanH Enroth
Jun 4, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C KupschY Gräser
Jun 28, 2017·International Journal of Dermatology·Mahmoud GhannoumLuis Leal
Oct 23, 2010·Journal of Basic Microbiology·Alireza KhosraviAli Masoudi-Nejad
Dec 21, 2010·The British Journal of Dermatology·C L AlexanderC Williams
Jan 12, 2021·International Journal of Veterinary Science and Medicine·Hassan Aboul-EllaHeidy Abo-Elyazeed

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