Validation of procedures for quantitative whole-body autoradiography using digital imaging

Journal of Pharmaceutical Sciences
P A ZaneS L Tripp

Abstract

The objective of this study was to demonstrate that tissue concentrations of radioactivity derived by digital analysis of autoradiograms were comparable to values derived from direct sampling and analysis of tissues. In addition, we describe the preparation and calibration of standards for use in quantitative whole-body autoradiography. For this study, three male Long-Evans hooded rats were administered 14C radioactivity intravenously. The animals were sectioned for whole-body autoradiography, with concomitant sampling of blood and 16 selected tissues. After 3 weeks of film exposure, the optical densities of the resulting autoradiograms were analyzed with a RAS3000 digital imaging system to estimate tissue concentrations of radioactivity. These concentrations were then compared with those obtained by direct analysis of the tissue samples. The concentrations derived from digital analysis of the autoradiograms were very highly correlated with those determined from direct tissue analysis (r = 0.956). Linear regression analysis yielded a straight line with a slope of 0.97 and a goodness of fit (r2) of 0.913. This analysis suggested that there is an approximate 1:1 correlation between concentration values determined by the two metho...Continue Reading

References

Aug 1, 1988·Journal of Neuroscience Methods·A P Davenport, M D Hall
Jan 1, 1987·International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes·A SchweitzerW Niederberger
May 1, 1988·Toxicology and Applied Pharmacology·I FandD G Deutsch
Apr 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K Bergman, L Albanus
Jan 1, 1985·Critical Reviews in Toxicology·P BénardA G Rico
Oct 1, 1983·Journal of Pharmaceutical Sciences·L E Gerlowski, R K Jain
Dec 1, 1982·The International Journal of Applied Radiation and Isotopes·F Keller, P G Waser

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Citations

Sep 18, 2002·Nuclear Medicine and Biology·Kun-Ju LinJeng-Jong Hwang
May 25, 2012·Drug and Chemical Toxicology·María Luisa Lucero, Andrew B Patterson
May 12, 2000·Regulatory Toxicology and Pharmacology : RTP·U BuschG Nehmiz
May 12, 2000·Regulatory Toxicology and Pharmacology : RTP·C GüntherU Täuber

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