Validation of reference genes for quantitative real-time PCR in Périgord black truffle (Tuber melanosporum) developmental stages

Phytochemistry
Osvaldo ZariviGiovanni Pacioni

Abstract

The symbiotic fungus Tuber melanosporum Vittad. (Périgord black truffle) belongs to the Ascomycota and forms mutualistic symbiosis with tree and shrub roots. This truffle has a high value in a global market and is cultivated in many countries of both hemispheres. The publication of the T. melanosporum genome has given researchers unique opportunities to learn more about the biology of the fungus. Real-time quantitative PCR (qRT-PCR) is a definitive technique for quantitating differences in transcriptional gene expression levels between samples. To facilitate gene expression studies and obtain more accurate qRT-PCR data, normalization relative to stable housekeeping genes is required. These housekeeping genes must show stable expression under given experimental conditions for the qRT-PCR results to be accurate. Unfortunately, there are no studies on the stability of housekeeping genes used in T. melanosporum development. In this study, we present a morphological and microscopical classification of the developmental stages of T. melanosporum fruit body, and investigate the expression levels of 12 candidate reference genes (18S rRNA; 5.8S rRNA; Elongation factor 1-alpha; Elongation factor 1-beta; α-tubulin; 60S ribosomal protein L...Continue Reading

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Citations

Mar 14, 2018·Cell Death Discovery·Osvaldo ZariviMichele Miranda
Apr 6, 2019·Scientific Reports·Xiaolin LiBo Zhang
Aug 1, 2020·Archives of Microbiology·Urszula Perlińska-LenartJoanna S Kruszewska

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