Validation of reliable and selective methods for direct determination of glyphosate and aminomethylphosphonic acid in milk and urine using LC-MS/MS

Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Pamela K JensenMark A McGuire

Abstract

Simple high-throughput procedures were developed for the direct analysis of glyphosate [N-(phosphonomethyl)glycine] and aminomethylphosphonic acid (AMPA) in human and bovine milk and human urine matrices. Samples were extracted with an acidified aqueous solution on a high-speed shaker. Stable isotope labeled internal standards were added with the extraction solvent to ensure accurate tracking and quantitation. An additional cleanup procedure using partitioning with methylene chloride was required for milk matrices to minimize the presence of matrix components that can impact the longevity of the analytical column. Both analytes were analyzed directly, without derivatization, by liquid chromatography tandem mass spectrometry using two separate precursor-to-product transitions that ensure and confirm the accuracy of the measured results. Method performance was evaluated during validation through a series of assessments that included linearity, accuracy, precision, selectivity, ionization effects and carryover. Limits of quantitation (LOQ) were determined to be 0.1 and 10 µg/L (ppb) for urine and milk, respectively, for both glyphosate and AMPA. Mean recoveries for all matrices were within 89-107% at three separate fortification l...Continue Reading

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Citations

Aug 20, 2016·Environmental Science and Pollution Research International·Shahla Hosseini Bai, Steven M Ogbourne
Jul 21, 2017·Journal of Animal Science·A L Van Eenennaam, A E Young
Dec 30, 2017·Food Additives & Contaminants. Part B, Surveillance·Otmar ZollerChristoph Geiser
Feb 1, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Angela SantilioArianna Giambenedetti
Jan 26, 2018·Critical Reviews in Food Science and Nutrition·Ranga Rao AmbatiSu Yuepeng
Mar 10, 2018·Environmental Health : a Global Access Science Source·S ParvezP D Winchester
Sep 10, 2019·Journal of Animal Science·John L ViciniKatherine A Karberg
Sep 29, 2019·Occupational and Environmental Medicine·Evangelia-Theano SmpokouBen Caplin
Jul 21, 2020·Scientific Reports·Marcella SpinaciGiovanna Galeati
Oct 5, 2020·Analytical and Bioanalytical Chemistry·Adrian A FrankeJennifer F Lai
Mar 20, 2021·Environment International·Maite De MaríaNancy D Denslow
Jun 8, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Martins JansonsHark Bahadur Karkee
Jul 9, 2021·Environmental Toxicology and Pharmacology·Rubén D Díaz-MartínMiguel Betancourt-Lozano
Aug 3, 2021·Environmental Research·Corina LesseurJia Chen
Aug 17, 2021·Comprehensive Reviews in Food Science and Food Safety·John L ViciniJohn T Swarthout

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