Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain

PLoS Neglected Tropical Diseases
Mathieu BangertIsrael Cruz

Abstract

Visceral leishmaniasis (VL), the most severe form of leishmaniasis, is endemic in Europe with Mediterranean countries reporting endemic status alongside a worrying northward spread. Serological diagnosis, including immunochromatographic test based on the recombinant antigen rK39 (rK39-ICT) and a direct agglutination test (DAT) based on the whole parasite antigen, have been validated in regions with high VL burden, such as eastern Africa and the Indian subcontinent. To date, no studies using a large set of patients have performed an assessment of both methods within Europe. We selected a range of clinical serum samples from patients with confirmed VL (including HIV co-infection), Chagas disease, malaria, other parasitic infections and negative samples (n = 743; years 2009-2015) to test the performance of rK39-ICT rapid test (Kalazar Detect Rapid Test; InBios International, Inc., USA) and DAT (ITM-DAT/VLG; Institute of Tropical Medicine Antwerp, Belgium). An in-house immunofluorescence antibody test (IFAT), was included for comparison. Estimated sensitivities for rK39-ICT and DAT in HIV-negative VL patients were 83.1% [75.1-91.2] and 84.2% [76.3-92.1], respectively. Sensitivity was reduced to 67.3% [52.7-82.0] for rK39 and increa...Continue Reading

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Citations

Jul 19, 2019·PLoS Neglected Tropical Diseases·Mariana Lourenço FreireGláucia Cota
Jan 9, 2020·Expert Review of Anti-infective Therapy·Francesca F NormanRogelio López-Vélez
May 8, 2020·Parasite Immunology·Awanish KumarMukesh Samant
Aug 29, 2019·BMC Infectious Diseases·Giovanny HerreraJuan David Ramirez
May 19, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Shivani ThakurSukhbir Kaur
Mar 12, 2020·Frontiers in Cellular and Infection Microbiology·Maude F LévêqueChristelle Pomares
Oct 15, 2019·Frontiers in Cellular and Infection Microbiology·Fatima Conceição-Silva, Fernanda N Morgado
Mar 27, 2020·PLoS Neglected Tropical Diseases·Maude F LévêqueLaurence Lachaud
Nov 14, 2019·Trends in Parasitology·Ana V Ibarra-MenesesEugenia Carrillo
Oct 28, 2020·Microorganisms·Juliana Quero ReimãoAdriano Cappellazzo Coelho
Mar 12, 2021·Frontiers in Cellular and Infection Microbiology·Srija MoulikMitali Chatterjee
Apr 15, 2021·Cadernos de saúde pública·Fernanda Alvarenga Cardoso MedeirosAndreza Pain Marcelino
May 18, 2021·Frontiers in Medicine·Elis D da SilvaValéria R A Pereira
May 27, 2021·Revista Do Instituto De Medicina Tropical De São Paulo·Tália Machado de AssisGláucia Cota
Aug 25, 2021·European Journal of Pharmacology·Diksha KumariKuljit Singh
Sep 8, 2021·Microbial Genomics·Luz H PatinoJuan David Ramírez

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Methods Mentioned

BETA
PCR

Software Mentioned

epiR
R
STARD

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