Jan 5, 2016

Validation of SCT Methylation as a Hallmark Biomarker for Lung Cancers

Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer
Yu-An ZhangAdi Gazdar

Abstract

The human secretin gene (SCT) encodes secretin, a hormone with limited tissue distribution. Analysis of the 450k methylation array data in The Cancer Genome Atlas (TCGA) indicated that the SCT promoter region is differentially hypermethylated in lung cancer. Our purpose was to validate SCT methylation as a potential biomarker for lung cancer. We analyzed data from TCGA and developed and applied SCT-specific bisulfite DNA sequencing and quantitative methylation-specific polymerase chain reaction assays. The analyses of TCGA 450K data for 801 samples showed that SCT hypermethylation has an area under the curve (AUC) value greater than 0.98 that can be used to distinguish lung adenocarcinomas or squamous cell carcinomas from nonmalignant lung tissue. Bisulfite sequencing of lung cancer cell lines and normal blood cells allowed us to confirm that SCT methylation is highly discriminative. By applying a quantitative methylation-specific polymerase chain reaction assay, we found that SCT hypermethylation is frequently detected in all major subtypes of malignant non-small cell lung cancer (AUC = 0.92, n = 108) and small cell lung cancer (AUC = 0.93, n = 40) but is less frequent in lung carcinoids (AUC = 0.54, n = 20). SCT hypermethylat...Continue Reading

  • References48
  • Citations4

References

  • References48
  • Citations4

Citations

Mentioned in this Paper

Biological Markers
Genee-Wiedemann Syndrome
Pathogenic Aspects
Adenocarcinoma of Lung (Disorder)
Gene Expression Regulation, Neoplastic
Hydrogen sulfite
Carcinoid Tumor
The Cancer Genome Atlas
Pathogenesis
Protein Methylation

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