Validation of Size Estimation of Nanoparticle Tracking Analysis on Polydisperse Macromolecule Assembly

Scientific Reports
Ahram KimNam-Joon Cho

Abstract

As the physicochemical properties of drug delivery systems are governed not only by the material properties which they are compose of but by their size that they conform, it is crucial to determine the size and distribution of such systems with nanometer-scale precision. The standard technique used to measure the size distribution of nanometer-sized particles in suspension is dynamic light scattering (DLS). Recently, nanoparticle tracking analysis (NTA) has been introduced to measure the diffusion coefficient of particles in a sample to determine their size distribution in relation to DLS results. Because DLS and NTA use identical physical characteristics to determine particle size but differ in the weighting of the distribution, NTA can be a good verification tool for DLS and vice versa. In this study, we evaluated two NTA data analysis methods based on maximum-likelihood estimation, namely finite track length adjustment (FTLA) and an iterative method, on monodisperse polystyrene beads and polydisperse vesicles by comparing the results with DLS. The NTA results from both methods agreed well with the mean size and relative variance values from DLS for monodisperse polystyrene standards. However, for the lipid vesicles prepared ...Continue Reading

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Citations

Mar 5, 2020·Light, Science & Applications·Rubaiya HussainValerio Pruneri
Feb 28, 2021·Scientific Reports·Christian C E LuebbertMichael J W Johnston
Feb 12, 2021·Light, Science & Applications·Rubaiya HussainValerio Pruneri
May 1, 2021·Pharmaceutics·Jennifer CauzzoNataša Škalko-Basnet
Jun 17, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ritu GuptaHuan Xie

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Methods Mentioned

BETA
chemical
scanning
transmission
atomic force microscopy
size exclusion chromatography
light scattering
dynamic light scattering

Software Mentioned

FTLA
DLS
NTA

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