Validation of string test for diagnosis of Helicobacter pylori infections

Journal of Clinical Microbiology
B VelapatiñoD E Berg

Abstract

The method of recovering Helicobacter pylori DNA or viable cells absorbed on a string that a person has swallowed and that is retrieved an hour later (string test) should be a useful alternative to traditional analysis of cells or DNA obtained by endoscopy, which is invasive, uncomfortable, relatively costly, and ill-suited for community-based and pediatric studies. Here we assayed the sensitivity and validity of the string test versus conventional endoscopic biopsy for detecting and analyzing H. pylori infection. Forty-four people with gastric complaints were studied using both H. pylori culture and urease gene (ureB) PCR. H. pylori organisms cultured from strings and biopsy specimens from the same patients were fingerprinted by the randomly amplified polymorphic DNA (RAPD) method. Biopsy sections were also hematoxylin and eosin and silver stained for H. pylori detection. H. pylori was cultured from 80% of strings and detected by PCR from 91% of strings from participants whose biopsies had been H. pylori positive by culture, PCR, and/or histology. Strains recovered from strings and biopsy specimens yielded identical or closely related RAPD profiles in each of the 24 cases tested. We conclude that the string test is a useful me...Continue Reading

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Citations

Dec 21, 2012·Journal of Clinical Microbiology·Maria H R B GoncalvesLucia L B C Braga
Aug 23, 2008·World Journal of Gastroenterology : WJG·Ren-Xian TangJie Yan
Jul 21, 2009·European Journal of Pediatrics·Jeannette GuarnerSibylle Koletzko
Dec 6, 2007·Helicobacter·Alexander M Hirschl, Athanasios Makristathis
Aug 24, 2006·Helicobacter·Katarzyna Dzierzanowska-FangratDanuta Dzierzanowska
Mar 13, 2012·Helicobacter·Liviu A SicinschiBarbara G Schneider
Mar 7, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Monica ContrerasFabián Michelangeli
May 1, 2007·Current Therapeutic Research, Clinical and Experimental·Ayse Demet KayaSerdar Tuncer
Jul 22, 2015·The American Journal of Tropical Medicine and Hygiene·Monica ContrerasFabian Michelangeli
Aug 22, 2009·Current Opinion in Gastroenterology·Michael SelgradPeter Malfertheiner
Oct 31, 2015·The Pediatric Infectious Disease Journal·Margaret NansumbaMaryline Bonnet
Jul 25, 2013·Journal of Pediatric Gastroenterology and Nutrition·Richard N ArboledaPelayo Correa
Feb 9, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Parisa SabbaghSoheil Ebrahimpour

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