PMID: 16531770Mar 15, 2006Paper

Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
Alaina ArochoQiulu Pan

Abstract

Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder that is characterized by the presence of a reciprocal translocation between chromosomes 9 and 22 and results in the formation of the Philadelphia (Ph1) chromosome and is present in most of CML patients. The Ph1 chromosome forms a chimeric gene that encodes an abnormal P210 mRNA transcript in most CML patients. Surveillance for minimal residual disease by detection of BCR/ABL transcripts is currently done mostly by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). Quantitation of BCR/ABL transcripts can monitor tumor load and the outcome of therapy. Absolute quantification determines the input copy number of the transcript of interest, usually by plotting the amount of PCR product onto a standard curve based on serial dilutions of the same product cloned in plasmids. Relative quantification describes the change in expression of the target gene in the patient sample relative to that of a control transcript by using the 2-DeltaDeltaCt calculation. The results of real-time RT-PCR for BCR/ABL transcripts are often analyzed by using plasmid DNA standard curves. In the present study, 79 BCR/ABL transcript-positive samples from CML...Continue Reading

References

Feb 5, 2002·The Journal of Molecular Diagnostics : JMD·Adam Bagg
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 5, 2003·Seminars in Hematology·Tim Hughes, Susan Branford
Dec 12, 2003·Cancer·E Anders KolbPeter G Steinherz

❮ Previous
Next ❯

Citations

Jul 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniela CardinaleM Paola Costi
May 27, 2010·Journal of Experimental Botany·Einav Mayzlish-GatiHinanit Koltai
Feb 21, 2013·Nucleic Acids Research·Divita GargRebecca C Wade
Sep 13, 2012·Plant Physiology·Einav Mayzlish-GatiHinanit Koltai
Mar 31, 2009·Applied and Environmental Microbiology·Jungoh AhnHongweon Lee
Feb 23, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Davin MillerLawrence S Prince
Jul 10, 2012·BMC Gastroenterology·Tanielly Cristina Raiol SilvaRommel Rodríguez Burbano
May 19, 2011·Virology Journal·Shunchuan ZhangXiaoyue Chen
Aug 24, 2010·World Journal of Gastroenterology : WJG·Zhi-Na DunShu-Rui Xie
Jan 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sailaja KagitaRaghunadharao Digumarti
Jun 19, 2012·Clinical and Experimental Medicine·Mariana Ferreira LealRommel Rodríguez Burbano
Nov 10, 2013·Journal of Gastroenterology·Takayuki ShimizuKeiichi Kubota
Jul 3, 2015·Molecular Medicine Reports·Dongju ZhaoTaixin Shi
Mar 5, 2015·Molecular Medicine Reports·Xiaobing LiZhenjiang Liu
Apr 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sailaja KagitaRaghunadharao Digumarti
Feb 24, 2015·Nutrition and Cancer·Paola SignorelliAnna Caretti
Oct 28, 2016·Apoptosis : an International Journal on Programmed Cell Death·Han LiuMiaoxian Dong
Aug 23, 2016·Veterinary Surgery : VS·Matthew T BrokkenLinda A Dahlgren
Aug 11, 2007·The Journal of Molecular Diagnostics : JMD·Tong ZhangUNKNOWN Association for Molecular Pathology Hematopathology Subdivision
Jul 13, 2013·The American Journal of Sports Medicine·Shahril R ShaaraniJohn M O'Byrne
Sep 6, 2013·Journal of Dental Research·S S KimD W Hamilton
Jul 15, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jiang DuEnhua Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.