Validation of the CALUX bioassay as a screening and semi-quantitative method for PCDD/F levels in cow's milk

Journal of Hazardous Materials
I-Cheng ChouHsinyu Lee

Abstract

The objectives of this study were first to set up and validate the quality criteria of the Xenobiotic Detection Systems-chemical activated luciferase gene expression (CALUX) bioassay for the analysis of cow's milk samples spiked with polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs). The application of this bioassay was then tested by analyzing 28 commercially available pasteurized milk samples and comparing the data with the reference method for PCDD/F analysis. The CALUX criteria from the U.S. EPA and the European Union (EU) were calculated from 16 replaced spiked milk (SM) samples and 8 performance evaluation (PE) samples to validate the CALUX bioassay system. The CALUX bioassay criteria included control chart for quality control (QC) standards, recovery efficiency, and data comparability. The control chart for QC standards were both within the micro+/-2sigma range. The recovery efficiencies ranged from 60.4% to 106% with an average of 79.9% (relative standard deviation (RSD): 20.7%). The mean of data comparability (i.e., relative percent difference, RPD) between CALUX bioassay and high-resolution gas chromatography and high-resolution mass spectrometry (HRGC/HRMS) was 19.0% for SM samples. For th...Continue Reading

References

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Citations

May 22, 2013·Environmental Science and Pollution Research International·Kyu Tae LeeJong Seong Khim
Apr 29, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E WielogorskaL Connolly
Aug 13, 2009·Mass Spectrometry Reviews·Eric J Reiner
Jan 21, 2015·Journal of Environmental Quality·W C LinJ K Liu

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