PMID: 3773928Nov 1, 1986Paper

Validation of the SOS/Umu test with mutagenic complex mixtures

Mutation Research
W Z WhongT M Ong

Abstract

The SOS/Umu test, a rapid system for detecting genotoxic agents by monitoring SOS responses, was evaluated with the extracts of 8 mutagenic complex mixtures (airborne particles, coal dust, tobacco snuff, fried beef, fried shredded pork, airborne particles from polyurethane plants). In this system, the SOS function induced by genotoxicants is detected by a colorimetric measurement of beta-galactosidase in tester cells carrying a umuC-lacZ-fused gene on the plasmid. Results from the study show that a higher beta-galactosidase activity was found when the enzyme substrate and treated cells were added simultaneously into the enzyme reaction mixture and post-treatment dilution (10 X dilution with fresh medium) and incubation (for 2 h) were incorporated. The post-treatment dilution is necessary to reduce a possible false positive due to the color of test substances. The extracts of all mutagenic complex mixtures tested were found to induce dose-related SOS responses, indicating that the SOS/Umu test is potentially useful for the detection of mutagenic complex environmental mixtures.

References

Jun 1, 1985·Mutation Research·P Quillardet, M Hofnung
Feb 1, 1985·Scandinavian Journal of Work, Environment & Health·T M OngW Z Whong
May 1, 1983·Mutation Research·D M Maron, B N Ames
Jan 1, 1984·Journal of Toxicology and Environmental Health·W Z WhongT M Ong
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P QuillardetM Hofnung
Aug 1, 1982·Mutation Research·L F BjeldanesJ S Felton

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Citations

Jan 1, 1990·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·N BihariR K Zahn
Aug 12, 1996·Mutation Research·M el MzibriG Duménil
Jan 25, 2011·Microbial Biotechnology·Alva BiranShimshon Belkin
Dec 30, 2014·Bulletin of Environmental Contamination and Toxicology·Zhe TianHongyan Li
Jan 1, 1993·Environmental and Molecular Mutagenesis·A E McDanielsG N Stelma
May 10, 2005·Environmental and Molecular Mutagenesis·Georg ReifferscheidClaudia Schmid
Jan 1, 1988·Environmental and Molecular Mutagenesis·V S Houk, D M DeMarini
Jan 1, 1991·Environmental and Molecular Mutagenesis·J HeilR K Zahn
Jan 1, 1990·Environmental and Molecular Mutagenesis·A E McDanielsG N Stelma
Nov 11, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J Sanz-SerranoA Vettorazzi

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