Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory

Human Fertility : Journal of the British Fertility Society
Chey G DearingKevin S Lindsay

Abstract

Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Analyser (SCA) as a tool for routine sperm counting in a busy laboratory setting. The criteria examined were comparison with the Improved Neubauer and Leja 20-μm chambers, within and between field precision, sperm concentration linearity from a stock diluted in semen and media, accuracy against internal and external quality material, assessment of uneven flow effects and a receiver operating characteristic (ROC) analysis to predict fertility in comparison with the Neubauer method. This work demonstrates that SCA CASA technology is not a standalone 'black box', but rather a tool for well-trained staff that allows rapid, high-number sperm counting providing errors are identified and corrected. The system will produce accurate, linear, precise results, with less analytical variance than manual methods that correlate well against the Improved Ne...Continue Reading

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Citations

Jan 30, 2018·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Fereshteh DardmehParisa Gazerani
Mar 14, 2018·Reproduction, Fertility, and Development·Marc YesteMaria M Rivera Del Álamo
Sep 30, 2017·Human Fertility : Journal of the British Fertility Society·Chey G Dearing, Kevin S Lindsay
Sep 3, 2019·Andrologia·Benoit SchubertAndré Force
Nov 22, 2019·The World Journal of Men's Health·Christopher DouglasAshok Agarwal
Dec 31, 2020·Systems Biology in Reproductive Medicine·Fereshteh DardmehHiva Alipour
Feb 6, 2021·Journal of Gynecology Obstetrics and Human Reproduction·Jenna LammersThomas Fréour
Mar 20, 2021·Andrology·Changsheng DaiYu Sun

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