Validation of the sperm mobility assay in boars and stallions

Theriogenology
Jorge A Vizcarra, J Joe Ford

Abstract

The sperm mobility assay used in the present study measures the rate of sperm penetration in a biologically inert cell-separation solution (Accudenz). When a sample of sperm is overlaid in a cuvette containing Accudenz, sperm penetrate the solution and absorbance of the sample can be measured with a spectrophotometer. This assay has been successfully used to select chicken and turkey semen donors. We validated this assay for semen from boars and stallions. Absorbance was measured after overlaying fresh semen from each species in prefilled cuvettes for 1, 5, 10, 15, 20, and 40 min. There were no significant differences when sperm were incubated in prewarmed cuvettes at 37, 39, or 41 degrees C. However, a minimum concentration of 5x10(7) viable sperm/mL was needed to evaluate the rate of sperm penetration in boars. Absorbance was half-maximal at 5.4 and 14.1 min for boar and stallion sperm, respectively. Frequency analysis suggested a normal distribution of mobility values for boar sperm. There were positive correlations between mobility values and several computer-aided sperm analysis (CASA) parameters. In addition, there was medium repeatability for multiple ejaculates from single males. We concluded that the mobility assay can...Continue Reading

References

Jul 1, 1976·Journal of Reproduction and Fertility·E LinfordD L Stewart
Aug 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·B G Brackett, W L Williams
Nov 1, 1984·Poultry Science·S F Bilgili, J A Renden
Feb 25, 1998·Biology of Reproduction·D P Froman, A J Feltmann

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Citations

Apr 25, 2007·Annals of Medicine·Susie C HigginsMárta Korbonits
Sep 4, 2013·Theriogenology·Malgorzata A PozorMats H Troedsson
Jun 25, 2010·Animal Reproduction Science·S A OhM G Pang
Nov 26, 2009·Animal Reproduction Science·Nicolaia IaffaldanoSalvatore Passarella

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