Validation of transit-time ultrasound flow probes to directly measure portal blood flow in conscious rats

The American Journal of Physiology
M S D'AlmeidaD Lebrec

Abstract

Direct measurement of portal venous blood flow is technically difficult, yet crucial for accurate assessment of liver hemodynamic and metabolic functions. The aim of this investigation was to assess the feasibility of implanting transit-time ultra-sound (TTUS) perivascular flow probes on the portal vein of the rat and to validate this technique as a means of directly measuring portal blood flow in conscious rats. A TTUS flow probe was implanted on the portal veins of 10 rats. One week later, portal flow was measured under basal conditions in these rats by TTUS probes and after pharmacological manipulation of portal flow by intravenous injections of Glypressin or infusions of adenosine while the rats were conscious. Portal flow was simultaneously measured in the same rats using radioactive microspheres. Basal systemic hemodynamics, regional blood flows to splanchnic organs, and portal blood pressure were not significantly modified by the presence of the probe on the portal vein compared with a control group of rats not instrumented with flow probes. Basal portal flows measured by the TTUS and microsphere techniques were not different (20.6 +/- 2.6 and 17.6 +/- 1.3 ml/min). After Glypressin, portal flows measured by the TTUS and ...Continue Reading

Citations

Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S M JakobJ Takala
Aug 18, 2001·Journal of Applied Physiology·N VealP Calès
Dec 6, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ronald J JandacekPatrick Tso
Oct 28, 2004·American Journal of Physiology. Endocrinology and Metabolism·Cécile BosDaniel Tomé
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R Tatchum-TalomF H Khadour
Jul 22, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Virginia L BrooksYue Qi

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