Validation on milk and sprouts of EN ISO 16654:2001 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157

International Journal of Food Microbiology
Rosangela TozzoliStefano Morabito

Abstract

In 2006, the European Committee for standardisation (CEN)/Technical Committee 275 - Food analysis - Horizontal methods/Working Group 6 - Microbiology of the food chain (TC275/WG6), launched the project of validating the method ISO 16654:2001 for the detection of Escherichia coli O157 in foodstuff by the evaluation of its performance, in terms of sensitivity and specificity, through collaborative studies. Previously, a validation study had been conducted to assess the performance of the Method No 164 developed by the Nordic Committee for Food Analysis (NMKL), which aims at detecting E. coli O157 in food as well, and is based on a procedure equivalent to that of the ISO 16654:2001 standard. Therefore, CEN established that the validation data obtained for the NMKL Method 164 could be exploited for the ISO 16654:2001 validation project, integrated with new data obtained through two additional interlaboratory studies on milk and sprouts, run in the framework of the CEN mandate No. M381. The ISO 16654:2001 validation project was led by the European Union Reference Laboratory for Escherichia coli including VTEC (EURL-VTEC), which organized the collaborative validation study on milk in 2012 with 15 participating laboratories and that o...Continue Reading

Citations

Feb 3, 2019·Applied and Environmental Microbiology·Anniina JaakkonenMarjaana Hakkinen
Feb 20, 2019·Applied Microbiology and Biotechnology·Miloš RadosavljevićLjiljana Mojović
Jul 12, 2019·Journal of Oleo Science·Junmin JiXuede Wang
Dec 26, 2019·Biocontrol Science·Hajime TeramuraHirokazu Ogihara
Mar 7, 2020·International Journal of Environmental Research and Public Health·Dorota ZielińskaDanuta Kołożyn-Krajewska
Mar 4, 2020·Animals : an Open Access Journal From MDPI·Alessandra PelagalliSerena Calabrò
Dec 7, 2019·Foodborne Pathogens and Disease·Victoria GarridoMaría-Jesús Grilló
Jul 2, 2020·Risk Analysis : an Official Publication of the Society for Risk Analysis·Rita BernardoAna Rita Henriques
Aug 4, 2020·Journal of Applied Microbiology·A KolokotsaE Sazakli
May 19, 2020·Journal of Food Protection·LuÍs Augusto NeroCristina Constantino
Oct 13, 2019·Scientific Reports·Sara Khoshnoudi-Nia, Marzieh Moosavi-Nasab

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