Validation study of the in vitro skin irritation test with the LabCyte EPI-MODEL24

Alternatives to Laboratory Animals : ATLA
Hajime KojimaTakashi Omori

Abstract

A validation study on an in vitro skin irritation assay was performed with the reconstructed human epidermis (RhE) LabCyte EPI-MODEL24, developed by Japan Tissue Engineering Co. Ltd (Gamagori, Japan). The protocol that was followed in the current study was an optimised version of the EpiSkin protocol (LabCyte assay). According to the United Nations Globally Harmonised System (UN GHS) of classification for assessing the skin irritation potential of a chemical, 12 irritants and 13 non-irritants were validated by a minimum of six laboratories from the Japanese Society for Alternatives to Animal Experiments (JSAAE) skin irritation assay validation study management team (VMT). The 25 chemicals were listed in the European Centre for the Validation of Alternative Methods (ECVAM) performance standards. The reconstructed tissues were exposed to the chemicals for 15 minutes and incubated for 42 hours in fresh culture medium. Subsequently, the level of interleukin-1 alpha (IL-1 α) present in the conditioned medium was measured, and tissue viability was assessed by using the MTT assay. The results of the MTT assay obtained with the LabCyte EPI-MODEL24 (LabCyte MTT assay) demonstrated high within-laboratory and between-laboratory reproducib...Continue Reading

References

Sep 1, 2000·International Journal of Pharmaceutics·M PonecM Mommaas
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J H FentemP A Botham
Nov 9, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P PortesR Roguet
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C L CannonM Klausner

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Citations

Jun 5, 2020·Alternatives to Laboratory Animals : ATLA·Samara EberlinAdriano da Silva Pinheiro
Feb 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yulia Kaluzhny, Mitchell Klausner
Jun 2, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chih-Ching LiaoWannhsin Chen

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