PMID: 20650130Aug 1, 1995Paper

Validation study on five different cytotoxicity assays in Japan-an intermediate report

Toxicology in Vitro : an International Journal Published in Association with BIBRA
T OhnoHiroshi Ono

Abstract

In October 1992, the Japanese Society of Alternatives to Animal Experiments (JSAAE) organized a first-step inter-laboratory validation study on five Cytotoxicity assays: crystal-violet staining assay, neutral red uptake, MTT assay, colony formation, and lactate dehydrogenase release assay. This study is to clarify problems in organizing system of validation, protocols and intra- and inter-laboratory variation of ED(50) values on six representative chemicals determined with five cell lines in 45 laboratories, and thereby to evaluate practicability of the Cytotoxicity assays for further validation targetting to the Draize test. Two samples of one of the six chemicals were used to test intra-laboratory variation, so seven chemical preparations were sent to each laboratory after double-blind coding. More than 2300 data files were submitted to the organizing committee in 1993 and now they are under data cleaning and comprehensive analysis. Preliminary results calculated from hand-plotted figures were presented on a typical severe irritant, cetylpyridium chloride monohydrate.

References

Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K SaotomeM Umeda
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H ItagakiM Umeda
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K SasakiM Yamada
Sep 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T SasakiT Ohno

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Citations

Dec 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N LoprienoS Kato
Aug 26, 1998·Revista de saúde pública·A S CruzL F de Salles-Gomes
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Jun 1, 2004·Alternatives to Laboratory Animals : ATLA·Hiroshi Ono

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