Validity of acute and chronic tactile sensory testing after spinal cord injury in rats.

Experimental Neurology
Megan R DetloffD Michele Basso

Abstract

Spinal cord injury (SCI) impairs sensory systems causing allodynia. Measuring the development of allodynia in rodent models of SCI is challenging due to spinal shock and marked motor impairments. Assessment of SCI-induced allodynia is not standardized across labs, making interpretation of results difficult. Therefore, we validated sensory threshold assessment after SCI and developed a novel assessment of allodynia prior to motor recovery in a rat SCI model. One hundred fifty-six Sprague-Dawley rats received T8 laminectomy or mild to moderate SCI using the OSU SCI device (0.3 mm to 1.3 mm cord displacement). To determine tactile thresholds, von Frey hairs (VFH) were applied in Up-Down or ascending order to the dorsal or plantar hindpaw. The most efficient and valid procedures that maintain high sensitivity and specificity were identified. Ten Up-Down VFH applications yielded stable thresholds; reducing the risk of threshold decay and unnecessary exposure to painful stimuli. Importantly, distraction of SCI-rats with food revealed differential decay of thresholds than when distraction is not provided. The new test uses dorsal VFH stimulation and is independent of trunk or hindlimb control. Acute dorsal VFH thresholds collected bef...Continue Reading

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Citations

Jun 21, 2011·Journal of Neurotrauma·Eva Santos-NogueiraXavier Navarro
Dec 12, 2012·Journal of Neurotrauma·Megan Ryan DetloffJohn D Houlé
Apr 18, 2012·Journal of Visualized Experiments : JoVE·Megan Ryan DetloffD Michele Basso
Sep 10, 2013·The Spine Journal : Official Journal of the North American Spine Society·Ivana GrulovaDasa Cizkova
Mar 5, 2016·Frontiers in Veterinary Science·Sarah A Moore
Apr 16, 2013·The Veterinary Journal·B Duncan X Lascelles
Dec 3, 2016·Lasers in Medical Science·Suellen VeronezCarla Christina Medalha
Dec 9, 2017·Journal of Cellular Biochemistry·Paulina Nuñez-BadinezBrigitte van Zundert
Feb 2, 2018·Journal of Neurotrauma·Timothy D FawD Michele Basso
Sep 10, 2020·Advanced Healthcare Materials·Shaowei GuoShulamit Levenberg
Jan 1, 2020·European Journal of Pain : EJP·Sarah Louise ChristensenDavid M Kristensen
Jun 18, 2021·ACS Nano·Iftach ShlomyBen M Maoz
Oct 2, 2021·Frontiers in Pharmacology·Fangliang GuoWei Wang

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