Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death

Clinica Chimica Acta; International Journal of Clinical Chemistry
S TakeichiK Nakajima

Abstract

Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y = 1.03x + 229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.

References

Dec 1, 1990·Baillière's Clinical Endocrinology and Metabolism·A Hamsten
Apr 15, 1995·Thrombosis Research·R KnöflerA Takada
Dec 31, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·K NakajimaE Campos
Jan 1, 1997·International Journal of Legal Medicine·S TakeichiK Nakajima
Aug 14, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·H DoiH Yasue
Dec 24, 1960·British Medical Journal·J N GLANVILLE
Sep 1, 1961·Journal of Clinical Pathology·J B ENTICKNAP

Citations

Nov 19, 2010·Forensic Science International : Synergy·K NakajimaMasaki Q Fujita
May 3, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·K NakajimaAkira Tanaka
Aug 20, 2017·International Journal of Legal Medicine·Charlotte GirardCristian Palmiere

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