PMID: 9431461Feb 12, 1998Paper

Valinomycin induces apoptosis of ascites hepatoma cells (AH-130) in relation to mitochondrial membrane potential

Cell Structure and Function
Y InaiK Utsumi

Abstract

Valinomycin is a potassium ionophore, and is well known to cause the collapse of the mitochondrial membrane potential. It has been reported that loss of mitochondrial membrane potential is observed in the early stages of apoptosis induced by various agents. Thus, the effects of valinomycin on tumor cells were examined. Valinomycin induced uncoupling of respiration and depolarization of isolated mitochondria. Depolarization of intact mitochondria in AH-130 rat ascites hepatoma cells was also induced by valinomycin. Valinomycin induced apoptosis revealing the typical apoptotic characteristics such as fragmentation and ladder formation of DNA, shrinkage of cells, and formation of pycnotic nucleus. There was a correlation between the depolarization of mitochondria and DNA fragmentation. After depolarization of mitochondria, the activity of caspase-3-like protease but not caspase-1-like protease increased markedly. In contrast, this apoptosis did not involve the release of reactive oxygen species from mitochondria, increase in intracellular calcium concentration, or protein synthesis. In addition, anti-apoptotic members of the Bcl-2 family (Bcl-xL and Bcl-2) were not correlated with apoptosis. These results indicate that valinomycin...Continue Reading

Citations

Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P Yu, D W Choi
May 11, 2006·The Journal of Membrane Biology·E D BurgJ X-J Yuan
Oct 24, 2002·European Journal of Biochemistry·Yasuo ShinoharaHiroshi Terada
Jul 9, 2011·Apoptosis : an International Journal on Programmed Cell Death·Dario Domenico LofrumentoNicola Elio Lofrumento
Aug 31, 2011·Apoptosis : an International Journal on Programmed Cell Death·Barbara KleinHubert H Kerschbaum
Oct 6, 2007·Journal of Bioenergetics and Biomembranes·Vera V TeplovaMirja S Salkinoja-Salonen
Jul 28, 2009·International Journal of Cancer. Journal International Du Cancer·Steven S ChangJoseph A Califano
Jul 22, 2006·Neuroscience Letters·Rany AbdalahShan Ping Yu
Dec 13, 2005·Free Radical Biology & Medicine·Quan LiMasayasu Inoue
Mar 8, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Angelika BondzioRalf Einspanier
May 17, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RobaszkiewiczM Soszyński
Feb 26, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Anna JanaszewskaBarbara Klajnert
Sep 18, 2016·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Armen V GyulkhandanyanValery Leytin
Mar 13, 2014·Archives of Biochemistry and Biophysics·Agnieszka RobaszkiewiczMirosław Soszyński
Aug 3, 1999·European Journal of Biochemistry·R MikkolaM S Salkinoja-Salonen
Oct 6, 2007·The British Journal of Dermatology·M DendaP M Elias
Jan 2, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elizabeth RoundhillSusan Burchill
Feb 26, 1999·The Journal of Biological Chemistry·P GhafourifarC Richter
Mar 30, 2001·The Journal of Biological Chemistry·C TagliarinoD A Boothman
Oct 26, 2000·The Journal of Biological Chemistry·C D BortnerJ A Cidlowski
Dec 6, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carmelle V Remillard, Jason X-J Yuan
Feb 24, 2016·Molecular and Cellular Biochemistry·Swamy JagadishKanchugarakoppal S Rangappa
May 13, 2020·Journal of Cell Science·Priyanka S RanaMichael A Model
Oct 20, 2001·Molecular Pharmacology·C M LuetjensJ H Prehn
Mar 14, 2001·American Journal of Physiology. Cell Physiology·S KrickJ X Yuan
Jun 13, 2001·American Journal of Physiology. Cell Physiology·D EkhteraeJ X Yuan
May 3, 2019·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Zhe LiDavid D Nolte
May 26, 1999·FEMS Immunology and Medical Microbiology·K YokoyamaM Ohta

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