Valorisation of side streams from wheat milling and confectionery industries for consolidated production and extraction of microbial lipids

Food Chemistry
S TsakonaA A Koutinas

Abstract

Crude enzymes produced via solid state fermentation (SSF) using wheat milling by-products have been employed for both fermentation media production using flour-rich waste (FRW) streams and lysis of Rhodosporidium toruloides yeast cells. Filter sterilization of crude hydrolysates was more beneficial than heat sterilization regarding yeast growth and microbial oil production. The initial carbon to free amino nitrogen ratio of crude hydrolysates was optimised (80.2g/g) in fed-batch cultures of R. toruloides leading to a total dry weight of 61.2g/L with microbial oil content of 61.8% (w/w). Employing a feeding strategy where the glucose concentration was maintained in the range of 12.2-17.6g/L led to the highest productivity (0.32 g/L·h). The crude enzymes produced by SSF were utilised for yeast cell treatment leading to simultaneous release of around 80% of total lipids in the broth and production of a hydrolysate suitable as yeast extract replacement.

References

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Dec 3, 2014·Bioresource Technology·Xiaobing YangZongbao Kent Zhao

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Citations

Feb 10, 2018·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis
Nov 9, 2017·Journal of Applied Microbiology·M AthenakiS Papanikolaou
Sep 7, 2016·Engineering in Life Sciences·Eleni E KaramerouColin Webb
Oct 15, 2020·FEMS Microbiology Letters·Dimitris SarrisPanagiota Diamantopoulou

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