Valproic acid-mediated transcriptional regulation of human GM3 synthase (hST3Gal V) in SK-N-BE(2)-C human neuroblastoma cells

Acta Pharmacologica Sinica
Haw-Young KwonYoung-Choon Lee

Abstract

To investigate whether valproic acid (VPA) modulates human GM3 synthase (hST3Gal V) mRNA expression, as a part of ganglioside GM3 biosynthesis, in human neuroblastoma cells. Using RT-PCR and immunofluorescent confocal microscopy, we examined hST3Gal V mRNA and GM3 levels during VPA-induced differentiation of human neuroblastoma SK-N-BE(2)-C cells. We characterized the VPA-inducible promoter region within the hST3- Gal V gene using luciferase constructs carrying 5'-deletions of the hST3Gal V promoter. RT-PCR indicated that VPA-mediated hST3Gal V induction is transcriptionally regulated. Functional analysis of the 5'-flanking region of the hST3Gal V gene demonstrated that the -177 to -83 region, which contains a cAMP-responsive element (CRE) at -143, functions as the VPA-inducible promoter by actively binding CRE binding protein (CREB). In addition, sitedirected mutagenesis and electrophoretic mobility shift assay indicated that the CRE at -143 is crucial for the VPA-induced expression of hST3Gal V in SK-NBE( 2)-C cells. Our results isolated the core promoter region in the hST3Gal V promoter, a CRE at -143, and demonstrated that it is essential for transcriptional activation of hST3Gal V in VPA-induced SK-N-BE(2)-C cells. Subsequ...Continue Reading

References

Jan 1, 1980·Advances in Experimental Medicine and Biology·L Svennerholm
Jan 1, 1981·Annual Review of Biochemistry·S Hakomori
Mar 1, 1997·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·G ChenH K Manji
Jan 22, 1998·Anti-cancer Drugs·J CinatlD Schwabe
Jan 16, 1999·Brain Research. Molecular Brain Research·G ChenH K Manji
Jul 3, 1999·Trends in Biochemical Sciences·D De CesareP Sassone-Corsi
May 17, 2000·Neurochemistry International·C U Johannessen
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg
Oct 24, 2002·Biochimica Et Biophysica Acta·Sang-Wan KimYoung-Choon Lee
Feb 12, 2004·Cancer Research·Nadia GurvichPeter S Klein
Jun 23, 2004·Nature Reviews. Neuroscience·Michael A Rogawski, Wolfgang Löscher
Jan 8, 2005·Medicinal Research Reviews·Roman A BlahetaJindrich Cinatl
Feb 16, 2005·International Immunopharmacology·Wolf-Dietrich BeeckenRoman A Blaheta

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Citations

Jan 6, 2016·International Journal of Molecular Sciences·Hyun-Kyoung YoonYoung-Choon Lee
Aug 5, 2016·International Journal of Molecular Sciences·Hyun-Kyoung YoonYoung-Choon Lee
Dec 12, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Miri LeeYoung-Choon Lee

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