Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing.

Cells
Hanseul ParkJongpil Kim

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.

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Citations

Aug 8, 2021·International Journal of Molecular Sciences·Seung Hee ChoiSuk Weon Kim
Sep 11, 2021·Stem Cell Research & Therapy·Manoj Kumar K AzhagiriSaravanabhavan Thangavel

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Datasets Mentioned

BETA
GSM595518

Methods Mentioned

BETA
transgenic
transfection
histone acetylation
in vitro transcription
PCR
Assay
M13 sequencing
electrophoresis
immunoprecipitation
acetylation

Software Mentioned

Bowtie2
- OFFinder
Integrative Genomics Viewer ( IGV )
SPSS
Cas
OFFinder
ImageJ
HOMER

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