Value of phenotyping methods as an initial screening of Pseudomonas aeruginosa in epidemiologic studies

Infection
D C BergmansE E Stobberingh

Abstract

When studying the epidemiology of Pseudomonas aeruginosa, determination of the similarity of isolates is crucial. In the present study the distinctive capacity of four phenotyping methods (antibiotic susceptibility patterns, serotyping, phage-typing and outer membrane protein [OMP] profile analysis) was determined and compared to pulsed-field gel electrophoresis (PFGE) of enzyme restricted chromosomal DNA. In all, 91 isolates of P. aeruginosa were cultured from ten patients. Antibiotic susceptibility patterns were concordant for all isolates. Serotyping yielded five, phage-typing eight, OMP profile analysis nine and PFGE seven distinct types of P. aeruginosa. Compared to PFGE, the distinctive capacities were 89% (81/91) for serotyping, 87% (79/91) for phage-typing, and 90% (82/91) for OMP profile analysis. When serotyping results were different, PFGE types also were different (exclusiveness 100%). However, isolates with the same serotype may have various PFGE patterns. In contrast, isolates with similar PFGE patterns could have different phage-types or OMP types. For the study of isolates of P. aeruginosa, serotyping provides a good initial selection to reduce the number of isolates that need to be genotyped.

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Citations

Oct 6, 1999·American Journal of Respiratory and Critical Care Medicine·M J BontenE E Stobberingh
May 29, 2015·Biotechnology, Biotechnological Equipment·Samy SelimMohamed Abdel Aziz
Dec 23, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A L Freitas, A L Barth
Feb 16, 2008·Analytical and Bioanalytical Chemistry·Satoshi FujitaHiroo Iwata
Aug 10, 2010·Pastoral Psychology·Jacob A Belzen
Jul 28, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Balasundari Ramesh, Soma Guhathakurta

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