vanA genes in vancomycin-resistant clinical isolates of Oerskovia turbata and Arcanobacterium (Corynebacterium) haemolyticum

The Journal of Antimicrobial Chemotherapy
E G PowerG French

Abstract

We report the cloning and sequencing of vanA genes present in the high-level vancomycin- and teicoplanin-resistant clinical isolates Oerskovia turbata 892 and Arcanobacterium (Corynebacterium) haemolyticum 872. The presence of vanA was detected by Southern blotting and PCR and confirmed by DNA sequencing. vanA-like sequences were encoded on plasmids of 15 and 20 kb respectively. The A. haemolyticum 872 DNA sequence was identical to the published vanA sequence of vancomycin-resistant Enterococcus faecium BM4147, but the O. turbata 892 sequence showed three coding changes. Induction experiments indicated that vancomycin resistance in A. haemolyticum 872 and O. turbata 892 was constitutive. SDS-PAGE analysis of membrane proteins showed the presence of a c. 39 kD protein in both clinical isolates whose expression was unaltered in the presence of vancomycin, while a similar protein in E. faecium BM4147 was inducible. Since A. haemolyticum and O. turbata are naturally susceptible to vancomycin, the high-level constitutive resistance seen in these isolates appears to be mediated by vanA. This is the first report confirming the presence of vanA in genera other than Enterococcus.

Citations

Jun 1, 1997·Journal of Medical Microbiology·J G Hastings
Apr 3, 2001·International Journal of Antimicrobial Agents·A I VelaM A Moreno
Sep 24, 1999·International Journal of Antimicrobial Agents·M PuntorieriS Stefania
Mar 21, 2000·International Journal of Antimicrobial Agents·S Stefani, A Agodi
Mar 18, 2000·International Journal of Antimicrobial Agents·V Krcméry, A Sefton
Jun 1, 1997·Trends in Microbiology·T I NicasD K Braun
Apr 26, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D E LowR N Jones
Aug 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F C Tenover
Jan 5, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M G BonoraC Bordin
Jun 28, 2008·The Journal of Antimicrobial Chemotherapy·Lourdes Garcia-MiguraLars Bogø Jensen
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Luca GuardabassiPatrice Courvalin
Sep 25, 2008·Future Microbiology·Guido WernerWolfgang Witte
Mar 21, 1998·Emerging Infectious Diseases·B E Murray
Aug 27, 2002·Emerging Infectious Diseases·Diana PanessoCésar A Arias
Jan 1, 2011·The Australasian Medical Journal·Meher RizviAmir Bin Sabir
Jan 10, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M N AlmuzaraC Vay
Jan 1, 1997·The Journal of Hospital Infection·G G RaoD Kolokithas
Jan 17, 2002·Acta Microbiologica Et Immunologica Hungarica·F RozgonyiA Ghidán
Feb 16, 2006·The Journal of Antimicrobial Chemotherapy·Henrik HasmanLuca Guardabassi
Jun 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R FontanaG Cornaglia
Jul 14, 2020·Frontiers in Microbiology·Oleksandr YushchukFlavia Marinelli
Jun 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Mehdi Fatahi-Bafghi
Aug 4, 1998·Antimicrobial Agents and Chemotherapy·M LigozziR Fontana
Feb 27, 1999·Antimicrobial Agents and Chemotherapy·R J WillemsJ D van Embden
Sep 16, 1998·Antimicrobial Agents and Chemotherapy·C G MarshallG D Wright
Nov 2, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A SundsfjordP Courvalin
Jan 1, 1997·Clinical Microbiology Reviews·G FunkeK A Bernard
Aug 17, 2004·Infectious Disease Clinics of North America·Keith S KayeElias Abrutyn

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