PMID: 11931974Apr 5, 2002Paper

Vanadate-induced cell growth arrest is p53-dependent through activation of p21 in C141 cells

Journal of Inorganic Biochemistry
Zhuo ZhangXianglin Shi

Abstract

Vanadium is widely used in industry. It is a potent toxic agent and carcinogen. The mechanisms involved in its toxicity and carcinogenesis are still unclear. Improper cell growth is believed to be involved in cancer development. The present study investigated the regulation of p53 on vanadate-induced cell growth arrest using both p53 wild type C141 cells and p53 deficient embryo fibroblasts (p53 -/-). On vanadate stimulation, C141 cells exhibited a dose- and time-dependent S phase arrest as determined by DNA content analysis. In contrast, vanadate was unable to increase the percentage of S phase in p53 -/- cells. Luciferase assay showed that vanadate induced p53 activation in a dose- and time-dependent manner in p53 wild type C141 cells. Addition of pifithrin-alpha (PFT), a specific inhibitor of p53, reduced the activation of p53 with a concomitant decrease in growth arrest at S phase. Western blotting analysis demonstrated that vanadate caused a dose- and time-dependent increase of p21 level in C141 cells. Pretreatment of C141 cells with PFT decreased p21 expression induced by vanadate while the p21 expression did not vary in vanadate stimulated p53 -/- cells. The results obtained from the present study suggest that vanadate i...Continue Reading

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Citations

Feb 26, 2010·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Farida Louise Assem, Leonard Stephen Levy
Oct 24, 2013·Toxicology Mechanisms and Methods·Iwona Zwolak
Sep 7, 2016·Journal of Toxicology and Environmental Health. Part a·Jie YangGuo Shao
Dec 13, 2016·Drug and Chemical Toxicology·Rodrigo Anibal Mateos-NavaMario Agustín Altamirano-Lozano
Nov 16, 2014·Biological Trace Element Research·Jan KorbeckiDariusz Chlubek
Jul 11, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tridib ChakrabortyMalay Chatterjee
May 17, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ying FuKui Wang
Apr 30, 2021·Environmental Toxicology·Rodrigo Aníbal Mateos-NavaMario Agustín Altamirano-Lozano
Jan 5, 2011·Journal of Inorganic Biochemistry·Ellie HeintzeMatthew J Gage

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