PMID: 6167567Aug 10, 1981Paper

Vanadate inhibits protein degradation in isolated rat hepatocytes.

The Journal of Biological Chemistry
P O Seglen, P B Gordon

Abstract

Vanadate (10 mM) strongly inhibited endogenous protein degradation as well as the degradation of an exogenous, endocytosed protein (asialofetuin) in isolated rat hepatocytes. Protein synthesis and cellular viability were unaffected, but changes in cell morphology suggested some interference with cytoskeletal elements. The effect of vanadate was comparable to the effects of several other degradation inhibitors (lysosomotropic amines, leupeptin, vinblastine, amino acids, dimethylaminopurine riboside) known to inhibit the autophagic/lysosomal pathway of protein degradation. Vanadate inhibited proteolysis in a liver homogenate at pH 5, suggesting a direct effect upon the lysosomal proteinases.

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.