PMID: 6555085Sep 19, 1983

Vanadate stimulates tyrosine phosphorylation of two proteins in Raji human lymphoblastoid cell membranes

FEBS Letters
H S EarpR Dy

Abstract

A membrane fraction from Raji human lymphoblastoid cells exhibited tyrosine-specific kinase activity. Vanadate increased tyrosine phosphorylation up to 5-fold; serine and threonine phosphorylation were unchanged. The stimulation was detectable within 15 s at 0 degrees C and at concentrations of vanadate (0.3 and 1.0 microM) present in normal tissues and blood. The tyrosine phosphorylation of two substrates, M1 61 000 and 55 000, was dependent upon vanadate and incorporation into these substrates represented the majority of the vanadate-sensitive tyrosine phosphorylation.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P P Layne, V A Najjar
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M S Collett, R L Erikson
Feb 1, 1981·Molecular and Cellular Biology·J A Cooper, T Hunter
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Aug 1, 1982·Biochemical and Biophysical Research Communications·G SwarupD L Garbers
Oct 30, 1981·Biochemical and Biophysical Research Communications·G Carpenter
Jan 1, 1981·Current Topics in Cellular Regulation·T Ramasarma, F L Crane
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E CasnellieE G Krebs
May 1, 1965·Journal of Clinical Pathology·J V PULVERTAFT

Citations

Mar 1, 1994·Developmental and Comparative Immunology·D L Evans, L Jaso-Friedmann
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H S EarpG Y Gillespie
Dec 28, 1984·Biochemical and Biophysical Research Communications·A E NelR M Galbraith
Mar 24, 1993·Biochemical Pharmacology·T W MittagT Taniguchi
Mar 1, 1988·Journal of Cellular Physiology·R MontesanoL Orci

Related Concepts

African Burkitt's Lymphoma
Plasma Membrane
SDS-PAGE
Enzyme Activation
Cell Surface Proteins
Phosphorylation
Protein KINASE
Protein Tyrosine Kinase
Vanadyl
Vanadium

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