Abstract
To elucidate factors that contribute to the development of vancomycin resistance in methicillin-resistant Staphylococcus aureus (MRSA). Forty-nine MRSA isolates were subjected to passage selection with vancomycin to isolate mutants with reduced susceptibility to vancomycin. One mutant was chosen for detailed molecular and biochemical characterization. Five vancomycin-resistant mutants (vancomycin MICs, 6-12 mg/L) were obtained in vitro from five MRSA parent isolates. Upon acquisition of vancomycin resistance, all mutants showed a concomitant decrease in oxacillin resistance. In one particular MRSA strain, selection for vancomycin resistance repeatedly produced deletions and rearrangements, including loss of the mecA gene. Pleiotropic phenotypical changes, such as yellow pigment formation, loss of haemolysis, thickened cell wall, increased resistance to lysostaphin and reduced cell wall turnover were observed in this mutant. Acquisition of vancomycin resistance in one MRSA strain triggered mecA deletion suggesting that this deletion, coupled to other rearrangements and/or mutations, may be responsible for the increased vancomycin resistance phenotype.
Citations
Nov 26, 2013·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Jessica K OrtwineMichael J Rybak
Aug 30, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kevin W McConeghyKeith A Rodvold
Sep 7, 2006·Antimicrobial Agents and Chemotherapy·Bruno Périchon, Patrice Courvalin
Jan 24, 2008·Antimicrobial Agents and Chemotherapy·Michael J NotoGordon L Archer
Mar 21, 2008·Journal of Clinical Microbiology·Patrice FrancoisJacques Schrenzel
Aug 19, 2007·Journal of Clinical Microbiology·Maria DaskalakiFernando Chaves
Jul 12, 2014·Genome Announcements·Stephanie A MatyiJohn E Gustafson
Apr 1, 2009·Antimicrobial Agents and Chemotherapy·Nagendra N MishraArnold S Bayer
Apr 7, 2009·Diagnostic Microbiology and Infectious Disease·Dewan Sakhawat BillalNoboru Yamanaka
Mar 13, 2016·The Journal of Antimicrobial Chemotherapy·Yu-Tzu LinLee-Jene Teng
Apr 1, 2016·Trials·Steven Y C TongUNKNOWN CAMERA2 study group and the Australasian Society for Infectious Diseases Clinical Research Network
Jun 15, 2007·Expert Opinion on Pharmacotherapy·Scott D Pope, Andrew M Roecker
Mar 18, 2011·International Journal of Antimicrobial Agents·A MartinsL Amaral
Oct 8, 2014·Clinical Therapeutics·Susan Hadley
Jun 30, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S MoneckeG Coombs
May 8, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sabrina Di GregorioMarta Mollerach
May 26, 2017·PloS One·Alejandra Ugarte TorresJohn Conly
Jan 26, 2017·Scientific Reports·Aihua WangChao Zhuo
Feb 12, 2005·The Journal of Antimicrobial Chemotherapy·Judith N SteenbergenFrancis P Tally
Jan 9, 2020·Microbial Genomics·Stefano G GiulieriDeborah A Williamson
Sep 6, 2005·The Journal of Antimicrobial Chemotherapy·Parham SendiWerner Zimmerli
May 17, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Warren RoseGeorge Sakoulas