Vancomycin tolerant, methicillin-resistant Staphylococcus aureus reveals the effects of vancomycin on cell wall thickening

PloS One
Vicenta Cázares-DomínguezJuan Xicohtencatl-Cortes

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important opportunistic pathogen that causes both healthcare- and community-acquired infections. An increase in the incidence of these infections may lead to a substantial change in the rate of vancomycin usage. Incidence of reduced susceptibility to vancomycin has been increasing worldwide for the last few years, conferring different levels of resistance to vancomycin as well as producing changes in the cell wall structure. The aim of the present study was to determine the effect of vancomycin on cell wall thickening in clinical isolates of vancomycin-tolerant (VT) MRSA obtained from pediatric patients. From a collection of 100 MRSA clinical isolates from pediatric patients, 12% (12/100) were characterized as VT-MRSA, and from them, 41.66% (5/12) exhibited the heterogeneous vancomycin-intermediate S. aureus (hVISA) phenotype. Multiplex-PCR assays revealed 66.66% (8/12), 25% (3/12), and 8.33% (1/12) of the VT-MRSA isolates were associated with agr group II, I, and III polymorphisms, respectively; the II-mec gene was amplified from 83.3% (10/12) of the isolates, and the mecIVa gene was amplified from 16.66% (2/12) of the isolates. Pulsed field electrophoresis (PFGE) finger...Continue Reading

References

Sep 17, 1998·The Journal of Antimicrobial Chemotherapy·J MayG French
Jan 9, 2003·Journal of Clinical Microbiology·Longzhu CuiKeiichi Hiramatsu
Jan 17, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patrick G P CharlesM Lindsay Grayson
Jul 1, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pamela A Moise-BroderRobert C Moellering
Feb 1, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P C Appelbaum
Apr 5, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fred C Tenover, Robert C Moellering
Jan 4, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alex SorianoJosep Mensa
Apr 9, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R N Jones
Feb 10, 2009·The Journal of Infectious Diseases·Stan Deresinski
Apr 3, 2009·Journal of Clinical Microbiology·Ana VindelUNKNOWN Spanish Group for the Study of Staphylococcus
Apr 24, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R K C FongA Kurup
Jul 11, 2012·Interdisciplinary Perspectives on Infectious Diseases·S Sujatha, Ira Praharaj
Jan 23, 2013·Medical Molecular Morphology·Kenji FukutsujiTamotsu Harada
Feb 15, 2013·Medical Molecular Morphology·Yukiyoshi HyoTamotsu Harada
May 17, 2014·Scandinavian Journal of Infectious Diseases·Eunice Mireya Borbón-EsquerMariana Del Rocío Ruiz-Briseño
Jun 3, 2014·Antimicrobial Resistance and Infection Control·Théodora Angèle AhoyoDidier Pittet
Jun 8, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M Stojanov, D S Blanc
Jun 25, 2014·Journal of Clinical and Diagnostic Research : JCDR·Meysam Hasannejad BibalanEzzat Allah Ghaemi
Jul 25, 2014·Antimicrobial Resistance and Infection Control·Alexander M AikenHajo Grundmann

❮ Previous
Next ❯

Citations

May 23, 2015·Frontiers in Microbiology·Vicenta Cázares-DomínguezJuan Xicohtencatl-Cortes
Dec 22, 2016·The Journal of Antimicrobial Chemotherapy·Nicholas S BrittMolly E Steed
Sep 25, 2019·Journal of Bacteriology·Giulia Orazi, George A O'Toole
Oct 20, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Adriana Medianeira RossatoPedro Alves d'Azevedo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
genotyping

Software Mentioned

NTSY

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.