Vangl2 regulates spermatid planar cell polarity through microtubule (MT)-based cytoskeleton in the rat testis

Cell Death & Disease
Haiqi ChenC Yan Cheng

Abstract

During spermatogenesis, developing elongating/elongated spermatids are highly polarized cells, displaying unique apico-basal polarity. For instance, the heads of spermatids align perpendicular to the basement membrane with their tails pointing to the tubule lumen. Thus, the maximal number of spermatids are packed within the limited space of the seminiferous epithelium to support spermatogenesis.  Herein, we reported findings that  elongating/elongated spermatids displayed planar cell polarity (PCP) in adult rat testes in which the proximal end of polarized spermatid heads were aligned uniformly across the plane of the seminiferous epithelium based on studies  using confocal microscopy and 3-dimensional (D) reconstruction of the seminiferous tubules.  We also discovered  that spermatid PCP was regulated by PCP protein Vangl2 (Van Gogh-like protein 2) since Vangl2 knockdown by RNAi was found to perturb spermatid PCP. More important, Vangl2 exerted its regulatory effects through changes in the organization of the microtubule (MT)-based cytoskeleton in the seminiferous epithelium. These changes were mediated via the downstream signaling proteins atypical protein kinase C ξ (PKCζ) and MT-associated protein (MAP)/microtubule affinity...Continue Reading

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M D WeingartenM W Kirschner
Dec 1, 1972·Journal of Ultrastructure Research·H P StanleyR F Wilkinson
Aug 1, 1980·The Journal of Urology·R P Amann, S S Howards
Jan 1, 1994·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S J Gwyther
Jul 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Hofmann
Apr 18, 2003·Biology of Reproduction·Yong-mei ChenC Yan Cheng
Apr 16, 2004·Current Biology : CB·Jonathan B HurovHelen Piwnica-Worms
Jun 7, 2005·Current Biology : CB·Tae Joo ParkJohn B Wallingford
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Y M ChenZ G Luo
Jun 15, 2007·Nature Cell Biology·Xian ZhangZhen-Ge Luo
Mar 13, 2008·Journal of Cell Science·Sarah DunnMichelle Peckham
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Elissa W P WongC Yan Cheng
Mar 20, 2010·Molecular Biology of the Cell·Lacramioara FabianJulie A Brill
May 4, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Deborah J Henderson, Bill Chaudhry
Jul 19, 2011·Developmental Cell·Ryan S GrayLilianna Solnica-Krezel
Aug 5, 2011·Development·Sang-Wook ChaJanet Heasman
Aug 30, 2011·Methods in Molecular Biology·Dolores D Mruk, C Yan Cheng
Jan 24, 2012·Ciência & saúde coletiva·Laura N VandenbergGilbert Schoenfelder
Nov 13, 2012·Current Topics in Developmental Biology·Paul N Adler
Nov 13, 2012·Current Topics in Developmental Biology·Helen May-Simera, Matthew W Kelley
Nov 13, 2012·Current Topics in Developmental Biology·Thomas J Carroll, Jing Yu
Mar 2, 2013·The Journal of Endocrinology·Elizabeth I TangC Yan Cheng
Apr 9, 2013·Seminars in Cell & Developmental Biology·Jérôme Ezan, Mireille Montcouquiol
Jul 11, 2013·Nature Reviews. Neuroscience·Fadel Tissir, André M Goffinet
Jul 25, 2013·American Journal of Physiology. Endocrinology and Metabolism·Hin-Ting WanC Yan Cheng
Sep 28, 2013·The Journal of Biological Chemistry·Bipul R AcharyaGeri Kreitzer
Jul 2, 2014·Endocrine-related Cancer·Jason HatakeyamaKermit L Carraway
Sep 30, 2014·International Review of Cell and Molecular Biology·Jocelyn A McDonald
Oct 29, 2014·The Journal of Cell Biology·Danelle Devenport
Dec 3, 2014·Cold Spring Harbor Perspectives in Medicine·Frederik TellkampCarien M Niessen
Sep 28, 2015·Trends in Biochemical Sciences·Katherine L GibbsGiampietro Schiavo

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Citations

Mar 25, 2020·Critical Reviews in Biochemistry and Molecular Biology·Linxi LiC Yan Cheng
Jul 11, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lingling WangC Yan Cheng
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiwen LiuC Yan Cheng
Feb 21, 2021·Nature Communications·Gabriel L GaleaAndrew J Copp
Jun 2, 2021·Seminars in Cell & Developmental Biology·Lingling WangC Yan Cheng

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Methods Mentioned

BETA
confocal microscopy
transfection
transfections
PCR

Software Mentioned

Olympus MicroSuite Five
Imaris Bitplane
Zeiss ZEN
Imaris Bitplane )
Autoquant X
Imaris

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