Vanillin inhibits PqsR-mediated virulence in Pseudomonas aeruginosa.

Food & Function
Nicholas MokSong Lin Chua

Abstract

Reduced efficacy of antibiotics in bacterial diseases is a global concern in clinical settings. Development of anti-virulence compounds which disarm bacterial virulence is an attractive therapeutic agent for complementary antibiotics usage. One potential target for anti-virulence compounds is quorum sensing (QS), the intercellular communication system in most pathogens, such as Pseudomonas aeruginosa. QS inhibitors (QSIs) can inhibit QS effectively, attenuate QS-mediated virulence, and improve host clearance of infections. While studies focused on developing homoserine-based las QSI, few targeted the quinolone-based pqs QS, which implicated host cytotoxicity and biofilm formation. It is imperative to develop novel anti-pqs-QS therapeutics for combinatorial antibiotic treatment of microbial diseases. We employed a gfp-based transcriptional pqs biosensor to screen a natural compounds library and identify vanillin (4-hydroxy-3-methoxybenzaldehyde), the primary phenolic aldehyde of vanilla bean. The vanillin inhibited pqs expression and its associated phenotypes, namely pyocyanin production and twitching motility in P. aeruginosa. Molecular docking results revealed that vanillin binds to the active site of PqsR, the PQS-binding res...Continue Reading

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Citations

Sep 29, 2020·Expert Opinion on Therapeutic Patents·Simone CarradoriFrancesca Sisto
Jan 19, 2021·Critical Reviews in Food Science and Nutrition·Zhiyun PengYong Zhao
Nov 19, 2020·Expert Opinion on Therapeutic Patents·Paolo GuglielmiGiulia Rotondi
May 4, 2021·Frontiers in Cellular and Infection Microbiology·R Frèdi LangendonkJoanne L Fothergill
Jun 22, 2021·Biosensors & Bioelectronics·Junchen LiaoBee Luan Khoo
Aug 28, 2021·International Journal of Molecular Sciences·Patrícia CoelhoLucinda J Bessa

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Methods Mentioned

BETA
biosensor
Fluorescence Imaging

Software Mentioned

Autodock Vina
GraphPad
Autodock Tools
Graphpad Prism
ImageJ

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