Van't Hoff and calorimetric enthalpies from isothermal titration calorimetry: are there significant discrepancies?

Biochemistry
J R HornK P Murphy

Abstract

The enthalpy of a reaction is most often determined through one of two means; it can be determined directly using calorimetry or indirectly by measuring the temperature dependence of the equilibrium constant (i.e., the van't Hoff method). Recently, discrepancies have been noted between the enthalpy measured by calorimetry, and the enthalpy determined by the van't Hoff method,. This has been suggested to indicate that the binding reaction is more complex than the simple one-to-one binding model used to describe the data. To better understand possible discrepancies between and, we have undertaken both experimental studies using isothermal titration calorimetry to measure the binding energetics of Ba(2+) binding 18-crown-6 ether and 2'-CMP binding RNase A, along with a simulation of a system involving a molecule in conformational equilibrium coupled with binding. We find that when experimental setup and analysis are correctly performed, no statistically significant discrepancies between and exist even for the linked system.

References


❮ Previous
Next ❯

Citations

Jun 28, 2011·Environmental Science and Pollution Research International·G García-RosalesE T Romero-Guzmán
Oct 2, 2007·Cell Biochemistry and Biophysics·Lee D HansenChristin T Choma
Sep 10, 2003·Analytical Biochemistry·Joel Tellinghuisen
Dec 5, 2002·Archives of Biochemistry and Biophysics·Gerardo Pérez-HernándezMarietta Tuena de Gómez-Puyou
Feb 13, 2003·Current Opinion in Structural Biology·Patricia C Weber, F Raymond Salemme
Nov 5, 2002·Current Opinion in Chemical Biology·Andrea VasellaMatthias Böhm
Jun 20, 2002·European Journal of Biochemistry·Peng WuNaoki Sugimoto
Mar 9, 2007·The Journal of Biological Chemistry·Alice KearneyP Anton van der Merwe
Dec 17, 2008·The Journal of Biological Chemistry·Nobuchika SuzukiTohru Kozasa
Mar 18, 2004·Oligonucleotides·Jean-Louis Mergny, Laurent Lacroix
Apr 19, 2013·Journal of Biochemistry·Tadessa DabaKuniyo Inouye
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Enrique García-HernándezMiguel Costas
Jun 16, 2010·Antimicrobial Agents and Chemotherapy·Suhman ChungStuart F J Le Grice
Jan 30, 2007·Journal of Bacteriology·Samuel A ShelburneDar-Chone Chow
Jun 25, 2008·Annual Review of Biophysics·Jonathan B Chaires
Mar 31, 2005·Annual Review of Physical Chemistry·Ninad V Prabhu, Kim A Sharp
Jul 8, 2009·International Journal of Molecular Sciences·Lina BaranauskieneDaumantas Matulis
Jun 6, 2014·Organic & Biomolecular Chemistry·Nasim FarahaniStephen J Loeb
Nov 3, 2015·Journal of Chemical Theory and Computation·Niel M HenriksenMichael K Gilson
Jun 30, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elliott J FrancoOliver Hofstetter
Apr 30, 2003·Biophysical Journal·Thomas PalmSarah E Hitchcock-DeGregori
Oct 1, 2005·Protein Science : a Publication of the Protein Society·Zhiwen XuHong Xue
Apr 11, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Martin TjahjonoMarc Garland
Aug 10, 2006·Journal of Molecular Recognition : JMR·Donald J Winzor, Craig M Jackson
Jan 24, 2006·Biopolymers·Peter J Mikulecky, Andrew L Feig
May 23, 2008·Journal of Molecular Recognition : JMR·Kathryn M ArmstrongBrian M Baker
May 1, 2007·Chemistry, an Asian Journal·Adam B BraunschweigJ Fraser Stoddart
Oct 28, 2005·Chembiochem : a European Journal of Chemical Biology·Nico J de MolRob M J Liskamp
Feb 14, 2015·Methods : a Companion to Methods in Enzymology·Olga Boudker, SeCheol Oh
Nov 11, 2009·Biotechnology Journal·L Selva RoselinWen-Yih Chen
Aug 11, 2004·Analytical Biochemistry·Mei-Chu LoGeorge Ellestad
Nov 22, 2005·Biophysical Journal·Binh NguyenW David Wilson
Nov 24, 2005·Biophysical Chemistry·Joel Tellinghuisen
Sep 29, 2004·Analytical Biochemistry·Joel Tellinghuisen
Feb 11, 2004·Analytical Biochemistry·Laura S Mizoue, Joel Tellinghuisen
Apr 29, 2015·Journal of Medicinal Chemistry·Stefan GeschwindnerPatrik Johansson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.