VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity

PloS One
Abigail SharrockJoanna L Hicks

Abstract

The chromosome of Mycobacterium tuberculosis (Mtb) contains a large number of Type II toxin-antitoxin (TA) systems. The majority of these belong to the VapBC TA family, characterised by the VapC protein consisting of a PIN domain with four conserved acidic residues, and proposed ribonuclease activity. Characterisation of five VapC (VapC1, 19, 27, 29 and 39) proteins from various regions of the Mtb chromosome using a combination of pentaprobe RNA sequences and mass spectrometry revealed a shared ribonuclease sequence-specificity with a preference for UAGG sequences. The TA complex VapBC29 is auto-regulatory and interacts with inverted repeat sequences in the vapBC29 promoter, whereas complexes VapBC1 and VapBC27 display no auto-regulatory properties. The difference in regulation could be due to the different properties of the VapB proteins, all of which belong to different VapB protein families. Regulation of the vapBC29 operon is specific, no cross-talk among Type II TA systems was observed. VapC29 is bacteriostatic when expressed in Mycobacterium smegmatis, whereas VapC1 and VapC27 displayed no toxicity upon expression in M. smegmatis. The shared sequence specificity of the five VapC proteins characterised is intriguing, we pr...Continue Reading

References

Oct 8, 2003·Nucleic Acids Research·Ann H Y KwanMerlin Crossley
Mar 24, 2004·Nature Reviews. Microbiology·Graham R StewartDouglas B Young
Feb 3, 2005·Nucleic Acids Research·Marian C J BlokpoelBrian D Robertson
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
Jan 12, 2007·Current Opinion in Microbiology·Neeraj Dhar, John D McKinney
May 30, 2009·PloS One·Dany J V BesteJohnjoe McFadden
Sep 30, 2010·The Journal of Biological Chemistry·Ling ZhuMasayori Inouye
Nov 3, 2010·Protein Engineering, Design & Selection : PEDS·Vickery L ArcusGregory M Cook
Jan 7, 2011·PloS One·Ghader BashiriEdward N Baker
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristoffer S Winther, Kenn Gerdes
Feb 23, 2012·The Journal of Biological Chemistry·Jared D SharpNancy A Woychik
Mar 1, 2012·Journal of Bacteriology·Joanna L McKenzieGregory M Cook
Nov 6, 2012·Nucleic Acids Research·Tige R RustadDavid R Sherman
Jan 25, 2013·Molecular & Cellular Proteomics : MCP·Jakob AlbrethsenIda Rosenkrands
Apr 5, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Chen-Yuan ChiangDonald A Enarson
Nov 15, 2013·Nature Communications·Kristoffer S WintherKenn Gerdes
May 14, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H EsmailR J Wilkinson
Sep 8, 2016·Nucleic Acids Research·Kristoffer WintherKenn Gerdes

❮ Previous
Next ❯

Citations

Dec 31, 2019·Protein Science : a Publication of the Protein Society·Emma S V Andrews, Vickery L Arcus
May 5, 2020·Frontiers in Genetics·Dukas Jurėnas, Laurence Van Melderen
Feb 27, 2021·Frontiers in Cellular and Infection Microbiology·Masanori AsaiSandra M Newton

❮ Previous
Next ❯

Methods Mentioned

BETA
affinity purification
PCR
gel filtration
electrophoresis
environmental stress

Software Mentioned

pBLAST
BLAST
ProtParam
TrASH

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.