Vapor-phase hydrogen peroxide as a surface decontaminant and sterilant.

Applied and Environmental Microbiology
N A Klapes, D Vesley

Abstract

The feasibility of utilizing vapor-phase hydrogen peroxide (VPHP) as a surface decontaminant and sterilant was evaluated in a centrifuge application. The prototype VPHP decontamination system, retrofitted into a Beckman L8-M ultracentrifuge, was designed to vaporize a 30% (wt/wt) solution of aqueous hydrogen peroxide continuously injecting and withdrawing VPHP in a deep-vacuum flow-through system. VPHP cycles of 4, 8, 16, and 32 min were examined for cidal activity against spores of Bacillus subtilis subsp. globigii and Bacillus stearothermophilus. Spore inocula (approximately 10(6)/coupon) were dried onto 0.5-in. (1.27-cm)-square stainless-steel coupons, and coupons were suspended in the centrifuge chamber, the space between the refrigeration can and the barrier ring (inner gap), and the space between the barrier ring and the vacuum ring (outer gap). At a chamber temperature of 4 degrees C, B. subtilis subsp. globigii spores were inactivated within 8 min, while inactivation of spores located in the outer gap at 27 degrees C required 32 min. The elevated temperature and high surface area/volume ratios in the outer gap may serve to decompose the gas more rapidly, thus reducing cidal efficacy. Of the two test spores, B. stearothe...Continue Reading

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Citations

Sep 21, 2006·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Sarah RobertsGraham J Sewell
May 17, 2005·Letters in Applied Microbiology·A KahnertS H E Kaufmann
Apr 12, 2012·Applied and Environmental Microbiology·T PottageA M Bennett
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Apr 26, 2012·The Journal of Antimicrobial Chemotherapy·Ezra LinleyJean-Yves Maillard
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Mar 20, 2021·American Journal of Infection Control·Jennifer Sanguinet, Charles Edmiston
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Aug 30, 2008·Environmental Science & Technology·Matthew F VerceTina M Carlsen
Feb 15, 2011·Infectious Disease Clinics of North America·William A Rutala, David J Weber

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