Var transcription profiling of Plasmodium falciparum 3D7: assignment of cytoadherent phenotypes to dominant transcripts

Malaria Journal
Uta GölnitzGerhard Wunderlich

Abstract

Cytoadherence of Plasmodium falciparum-infected red blood cells is mediated by var gene-encoded P. falciparum erythrocyte membrane protein-1 and host receptor preference depends in most cases on which of the 50-60 var genes per genome is expressed. Enrichment of phenotypically homogenous parasites by panning on receptor expressing cells is fundamental for the identification of the corresponding var transcript. P. falciparum 3D7 parasites were panned on several transfected CHO-cell lines and their var transcripts analysed by i) reverse transcription/PCR/cloning/sequencing using a universal DBLalpha specific oligonucleotide pair and ii) by reverse transcription followed by quantitative PCR using 57 different oligonucleotide pairs. Each cytoadherence selected parasite line also adhered to untransfected CHO-745 cells and upregulation of the var gene PFD995/PFD1000c was consistently associated with cytoadherence to all but one CHO cell line. In addition, parasites panned on different CHO cell lines revealed candidate var genes which reproducibly associated to the respective cytoadherent phenotype. The transcription profile obtained by RT-PCR/cloning/sequencing differed significantly from that of RT-quantitative PCR. Transfected CHO ...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D EskoW H Taylor
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·D I BaruchB L Pasloske
Nov 5, 1997·The American Journal of Tropical Medicine and Hygiene·C NewboldK Marsh
Sep 25, 1999·The American Journal of Tropical Medicine and Hygiene·S J RogersonM E Molyneux
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A BuffetA Scherf
Dec 29, 1999·Molecular and Biochemical Parasitology·H M TaylorC I Newbold
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J D SmithL H Miller
May 30, 2001·Molecular and Biochemical Parasitology·R NoviyantiJ C Reeder
Feb 8, 2002·Nature·Louis H MillerOgobara K Doumbo
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dror I BaruchWilliam E Collins
Jul 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jennifer PetersQin Cheng
Nov 13, 2002·Experimental Parasitology·Paulo Afonso NogueiraLuiz Hildebrando Pereira-da-Silva
Aug 16, 2003·Microbes and Infection·Irwin W ShermanEnrique Winograd
Mar 5, 2004·The Journal of Infectious Diseases·Kesinee ChotivanichNicholas J White
Jul 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul HorrocksChris I Newbold
Mar 31, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stuart A RalphArtur Scherf
Jun 18, 2005·The Journal of Infectious Diseases·Nicaise G Tuikue NdamPhilippe Deloron
Sep 3, 2005·Revista Do Instituto De Medicina Tropical De São Paulo·Gerhard WunderlichLuiz H Pereira-da-Silva

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Citations

Aug 18, 2009·Molecular and Biochemical Parasitology·Fernanda Janku Cabral, Gerhard Wunderlich
Aug 8, 2014·Memórias do Instituto Oswaldo Cruz·Alessandra Sampaio Bassi FratusGerhard Wunderlich
May 8, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nicaise Tuikue NdamPhilippe Deloron
Jul 6, 2020·Communications Biology·Radoslaw Igor OmelianczykPeter R Preiser
Jun 21, 2018·Scientific Reports·Rosana Beatriz Duque AraujoGerhard Wunderlich
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Luis Carlos Salazar AlvarezStefanie Costa Pinto Lopes
Jul 17, 2021·Frontiers in Cellular and Infection Microbiology·Eliana F G CubillosGerhard Wunderlich

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Methods Mentioned

BETA
PCR
transfection

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