Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes

Veterinary Microbiology
Eva ForsgrenIngemar Fries

Abstract

There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N=4) and field isolates (N=4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantification of the pathogen.

References

Jun 9, 2004·EMBO Reports·Zdena Palková
Mar 4, 2006·International Journal of Systematic and Evolutionary Microbiology·Elke GenerschIngemar Fries

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Citations

Feb 2, 2012·Biotechnology Letters·Zhenhua HuoRuifu Zhang
Jun 21, 2011·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Zenaida M De GuzmanChitho P Feliciano
Nov 17, 2009·Journal of Invertebrate Pathology·Elke Genersch
Jun 16, 2010·Environmental Microbiology·Dieter BehrensRobin F A Moritz
May 2, 2015·Archives of Microbiology·Douglas W Dingman
Aug 20, 2011·Current Microbiology·Angela Di PintoGiuseppina Tantillo
Aug 28, 2020·Frontiers in Microbiology·Ewa Jończyk-MatysiakAndrzej Górski
Sep 7, 2021·Frontiers in Veterinary Science·Jana BiováJiří Danihlík

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