Variability in synovial inflammation in rheumatoid arthritis investigated by microarray technology

Arthritis Research & Therapy
Johan LindbergJoakim Lundeberg

Abstract

In recent years microarray technology has been used increasingly to acquire knowledge about the pathogenic processes involved in rheumatoid arthritis. The present study investigated variations in gene expression in synovial tissues within and between patients with rheumatoid arthritis. This was done by applying microarray technology on multiple synovial biopsies obtained from the same knee joints. In this way the relative levels of intra-patient and inter-patient variation could be assessed. The biopsies were obtained from 13 different patients: 7 by orthopedic surgery and 6 by rheumatic arthroscopy. The data show that levels of heterogeneity varied substantially between the biopsies, because the number of genes found to be differentially expressed between pairs of biopsies from the same knee ranged from 6 to 2,133. Both arthroscopic and orthopedic biopsies were examined, allowing us to compare the two sampling methods. We found that the average number of differentially expressed genes between biopsies from the same patient was about three times larger in orthopedic than in arthroscopic biopsies. Using a parallel analysis of the tissues by immunohistochemistry, we also identified orthopedic biopsies that were unsuitable for gen...Continue Reading

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Citations

Sep 26, 2009·Molecular Human Reproduction·Mélanie HamelMarc-André Sirard
Dec 4, 2012·Rheumatology·Jean-Luc DavignonAlain Cantagrel
Dec 1, 2006·Arthritis Research & Therapy·Johan LindbergJoakim Lundeberg
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Methods Mentioned

BETA
biopsies
laser
biopsy
biospies
in vitro transcription
PCR
chip

Software Mentioned

GenePix
aroma
Expression Analysis Systematic Explorer ( EASE )
bioconductor
GO
R
MEV
EASE =
LIMMA
EASE

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