Variability of intracellular pH within individual populations of SS and AA erythrocytes

British Journal of Haematology
A A KaperonisS Chien

Abstract

Individual populations of AA and SS erythrocytes were fractionated according to cell density by centrifugation, and the fractions analysed for intracellular pH (PHi), the mole ratio of 2,3-diphosphoglycerate to haemoglobin (DPG:Hb), and cell concentration of haemoglobin (MCHC). The pHi of SS erythrocytes was consistently lower than that of AA erythrocytes throughout the density range, and the lowest pHi of both cell types (AA and SS) was found in cells with the highest density. As the highest density AA and SS erythrocytes are characterized by the lowest DPG:Hb values, their relatively low pHi cannot be ascribed to intracellular organic phosphate. Instead we propose that a redistribution of hydrogen ions across the membrane of both AA and SS erythrocytes is the ultimate result of progressive alterations in these membranes in vivo.

References

Mar 1, 1975·British Journal of Haematology·J A Warth, J F Desforges
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B Magdoff-FairchildJ F Bertles
Sep 1, 1976·British Medical Bulletin·A May, E R Huehns
Feb 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Dash, G J Brewer
Nov 1, 1977·British Journal of Haematology·J A WarthS Stolberg
Oct 1, 1975·The Journal of Clinical Investigation·H S Zarkowsky, R M Hochmuth
Jan 1, 1977·British Journal of Haematology·J Palek
Dec 1, 1978·British Journal of Haematology·B E GladerD G Nathan
May 1, 1974·Scandinavian Journal of Clinical and Laboratory Investigation·R D WoodsonJ C Detter
Apr 1, 1970·The Journal of Clinical Investigation·S ChienJ F Bertles
May 1, 1970·Analytical Biochemistry·Z B Rose, J Liebowitz
Aug 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·S ChienM I Gregersen

Citations

May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J R VotanoA Rich
Sep 26, 2003·Biophysical Journal·Zhi HuangDaniel B Kim-Shapiro
Jul 26, 2002·The Journal of Biological Chemistry·Xiuli XuDaniel B Kim-Shapiro
Sep 26, 2002·The Journal of Biological Chemistry·James P WillcocksKieran Clarke

Related Concepts

Anemia, Sickle Cell
Cell Separation
Centrifugation
Diphosphoglyceric Acids
Erythrocytes
Eryhem
Hydrogen-Ion Concentration

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.