Abstract
>Objective: Administration of purified human IgG from antiphospholipid syndrome patients has not consistently caused murine pregnancy loss despite the presence of significant anticardiolipin antibody (ACA) activity. We evaluated whether a correlation exists between ACA activity and the degree of fetal resorption in murine pregnancy and also determined whether pooled IgG from multiple ACA-positive patients increases the likelihood of fetal resorption compared with injection of single-donor IgG.Methods: Affinity chromatography followed by anisotropic ultrafiltration was used to extract and concentrate IgG from individual serum samples with and without ACA activity (ACA-positive IgG activity, 35-85 GPL versus ACA-negative IgG activity, <1 GPL) and from pooled aliquots derived from the same sera. On Day 8 of gestation, pregnant mice randomly received intraperitoneal injections of ACA-positive or ACA-negative, purified IgG (15 mg/mouse) or saline (1 ml). Laparotomies were performed on day 15, and uteri were harvested for gross evaluation and histologic study. Rates of fetal resorption were derived for each murine pregnancy (resorbed fetuses/resorbed fetuses + live pups) and compared between experimental groups.Results: A significant...Continue Reading