Variability of Potassium Channel Blockers in Mesobuthus eupeus Scorpion Venom with Focus on Kv1.1: AN INTEGRATED TRANSCRIPTOMIC AND PROTEOMIC STUDY.

The Journal of Biological Chemistry
Alexey I KuzmenkovEugene Grishin

Abstract

The lesser Asian scorpion Mesobuthus eupeus (Buthidae) is one of the most widely spread and dispersed species of the Mesobuthus genus, and its venom is actively studied. Nevertheless, a considerable amount of active compounds is still under-investigated due to the high complexity of this venom. Here, we report a comprehensive analysis of putative potassium channel toxins (KTxs) from the cDNA library of M. eupeus venom glands, and we compare the deduced KTx structures with peptides purified from the venom. For the transcriptome analysis, we used conventional tools as well as a search for structural motifs characteristic of scorpion venom components in the form of regular expressions. We found 59 candidate KTxs distributed in 30 subfamilies and presenting the cysteine-stabilized α/β and inhibitor cystine knot types of fold. M. eupeus venom was then separated to individual components by multistage chromatography. A facile fluorescent system based on the expression of the KcsA-Kv1.1 hybrid channels in Escherichia coli and utilization of a labeled scorpion toxin was elaborated and applied to follow Kv1.1 pore binding activity during venom separation. As a result, eight high affinity Kv1.1 channel blockers were identified, including ...Continue Reading

References

Apr 22, 1996·FEBS Letters·A K FilippovD A Brown
Nov 26, 1998·The Journal of Biological Chemistry·K KalmanK G Chandy
May 29, 2000·The Journal of Biological Chemistry·C LegrosO Pongs
Jun 23, 2000·Archives of Biochemistry and Biophysics·S K HoladayN R Krishna
Dec 1, 2000·Biochemical and Biophysical Research Communications·H Ouadid-AhidouchN Prevarskaya
Jan 14, 2001·The Journal of Biological Chemistry·Y V KorolkovaE V Grishin
Jan 4, 2003·The Journal of Biological Chemistry·Christine BeetonK George Chandy
May 28, 2003·Trends in Pharmacological Sciences·Ricardo C Rodríguez de la VegaLourival D Possani
Dec 17, 2003·The Biochemical Journal·Stéphanie MouhatJean-Marc Sabatier
Jun 23, 2004·Toxicon : Official Journal of the International Society on Toxinology·Ricardo C Rodríguez de la Vega, Lourival D Possani
Aug 25, 2004·Biophysical Journal·Payam AndalibStephen J Korn
Dec 14, 2004·The Biochemical Journal·Stéphanie MouhatJean-Marc Sabatier
Dec 14, 2004·CNS Drug Reviews·Keith C Hayes
Oct 18, 2006·Annals of Neurology·Evelyne BeraudPatrick J Cozzone
Nov 14, 2006·Toxicon : Official Journal of the International Society on Toxinology·Kenton J Swartz
Mar 31, 2007·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Diana Conte CamerinoJean-François Desaphy
Jan 25, 2008·The Biochemical Journal·Alexander A VassilevskiEugene V Grishin
May 13, 2008·Toxicon : Official Journal of the International Society on Toxinology·Yousra Abdel-MottalebJan Tytgat
Jul 24, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Oksana V NekrasovaMikhail P Kirpichnikov
Sep 11, 2008·Current Pharmaceutical Design·Stéphanie MouhatJean-Marc Sabatier
Sep 23, 2008·Biochemical and Biophysical Research Communications·Najwa AbbasMarie-France Martin-Eauclaire
Nov 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ricardo C Rodríguez de la VegaLourival D Possani
Dec 2, 2009·Nature Reviews. Drug Discovery·Heike WulffLuis A Pardo
Dec 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zoltan TakacsSteve A N Goldstein
Dec 17, 2009·Methods in Molecular Biology·Alexander A VassilevskiEugene V Grishin
Oct 5, 2010·Molecular & Cellular Proteomics : MCP·Shunyi ZhuJan Tytgat
Mar 4, 2011·Journal of Veterinary Science·Soo Hwa JangSo Yeong Lee
Mar 24, 2011·Future Medicinal Chemistry·Georg C TerstappenRavikumar Peri
Apr 7, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Bin GaoShunyi Zhu
Oct 8, 2011·Toxicon : Official Journal of the International Society on Toxinology·V Quintero-HernándezL D Possani
Jan 13, 2012·The Journal of Biological Chemistry·Alma Leticia SaucedoFederico del Río-Portilla

❮ Previous
Next ❯

Citations

Jul 28, 2016·Toxins·Andreas Hougaard LaustsenMikael Engmark
Sep 19, 2016·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·O V NekrasovaA V Feofanov
Sep 22, 2016·Scientific Reports·Alexey I KuzmenkovAlexander A Vassilevski
Dec 5, 2016·Journal of Biotechnology·Oksana NekrasovaAlexey Feofanov
Mar 22, 2018·PloS One·Ursula Castro de OliveiraInácio de Loiola Meirelles Junqueira-de-Azevedo
Feb 16, 2016·Biochemistry. Biokhimii︠a︡·A I KuzmenkovA A Vassilevski
Mar 19, 2016·British Journal of Clinical Pharmacology·Barbara MolesiniTiziana Pandolfini
Aug 1, 2020·Frontiers in Pharmacology·Andrei M GigolaevAlexander A Vassilevski
Mar 4, 2020·Toxicon : Official Journal of the International Society on Toxinology·Laura L Valdez-VelázquezLourival D Possani
Jul 17, 2018·Toxicon : Official Journal of the International Society on Toxinology·Micaiah J WardGunnar S Nystrom
Jun 7, 2021·Biochemical Pharmacology·Ksenia S KudryashovaAlexey V Feofanov

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.