Variable effects of 3 different chondroitin sulfate compounds on human osteoarthritic cartilage/chondrocytes: relevance of purity and production process

The Journal of Rheumatology
Steeve Kwan TatJ Martel-Pelletier

Abstract

During osteoarthritis (OA), the altered metabolism of cartilage involves proinflammatory factors and matrix metalloprotease (MMP) activity. Studies showed that chondroitin sulfate (CS) may exert a positive effect on the cartilage. Because of differences in CS in terms of purity and the production/purification process, we compared the effects of 3 different types of CS on human OA cartilage. Three types of CS were tested: CS1 (porcine, purity 90.4%), CS2 (bovine, purity 96.2%), and CS3 (bovine, purity 99.9%). Treatment with CS at 200 and 1000 microg/ml was performed on human OA cartilage explants in the presence/absence of interleukin 1ss (IL-1ss), and the protein modulations of factors including prostaglandin E(2) (PGE(2)), IL-6, and MMP-1 measured by ELISA. The CS effect on the expression of collagen type II was also investigated on OA chondrocytes using quantitative polymerase chain reaction. In the presence of IL-1ss, CS2 at 1000 microg/ml significantly inhibited IL-6 and PGE(2) production, and CS3 at 200 microg/ml markedly reduced the level of IL-6. CS1 was much less efficient at reducing the catabolic markers and in the absence of IL-1ss, it significantly increased IL-6 and MMP-1. IL-1ss significantly inhibited the gene ex...Continue Reading

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Citations

Aug 21, 2013·Current Rheumatology Reports·Yves Henrotin, Cécile Lambert
Dec 1, 2010·Therapeutic Advances in Musculoskeletal Disease·Yves HenrotinCecile Lambert
Sep 4, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuanhu ZhouJulie Glowacki
Jul 1, 2012·Carbohydrate Polymers·Riccardo A A MuzzarelliAntonio Gigante
May 20, 2016·Glycoconjugate Journal·Mamta BishnoiSanjay K Jain
Jun 11, 2014·Scientific Reports·Valentina CalamiaFrancisco J Blanco
Mar 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Johanne Martel-PelletierJean-Pierre Pelletier
Jun 28, 2019·Aging Clinical and Experimental Research·Germain HonvoJean-Yves Reginster
Dec 29, 2020·Carbohydrate Polymers·Qingshan ShenAurore Richel
Feb 8, 2021·Drug Discovery Today·Sarita Mishra, Munia Ganguli
Jun 14, 2021·International Journal of Biological Macromolecules·Huimin PangShu Jie Li

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