Variable expression of cystatin C in cultured trans-differentiating rat hepatic stellate cells

World Journal of Gastroenterology : WJG
Axel M GressnerRalf Weiskirchen

Abstract

To study the expression of cystatin C (CysC), its regulation by transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factor (PDGF) and the potential interference of CysC with TGF-beta1 signaling in this special cell type. We evaluated the CysC expression in cultured, profibrogenic hepatic stellate cells and trans-differentiated myofibroblasts by Northern and Western blotting and confocal laser scanning microscopy. CysC was increased significantly in the course of trans-differentiation. Both TGF-beta1 and PDGF-BB suppressed CysC expression. Furthermore, CysC secretion was induced by the treatment with TGF-beta1. Although CysC induced an increased binding affinity of TGF-beta receptor type III (beta-glycan) as assessed by chemical cross-linking with [125I]-TGF-beta1, it did not modulate TGF-beta1 signal transduction as shown by evaluating the Smad2/3 phosphorylation status and [CAGA]-MLP-luciferase reporter gene assay. Interestingly, the shedding of type III TGF-beta receptor beta-glycan was reduced in CysC-treated cells. Our data indicated that CysC expression was upregulated during trans-differentiation. Increased CysC levels in the serum of patients suffering from liver diseases are at least partially due t...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
Oct 15, 1977·Experimental Cell Research·D L KnookE C Sleyster
Aug 1, 1990·Journal of Biochemistry·Y MurawakiC Hirayama
Oct 30, 1990·Biochemical and Biophysical Research Communications·M SolemD Barnes
Jun 1, 1990·The Biochemical Journal·M AbrahamsonA Grubb
Sep 10, 1988·Lancet·A PalsdottirO Jensson
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SchäferA M Gressner
Apr 30, 1984·Biochemical and Biophysical Research Communications·A J BarrettA Grubb
Jan 13, 1984·Biochemical and Biophysical Research Communications·J BrzinW Machleidt
Nov 5, 1999·The Journal of Clinical Investigation·G P ShiH A Chapman
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S DooleyA M Gressner
Aug 17, 2001·European Journal of Gastroenterology & Hepatology·M TakeuchiT Hayakawa
Oct 27, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H FehrenbachA M Gressner
Jan 23, 2002·Molecular Reproduction and Development·Suzanne AfonsoBruce Babiarz
Apr 4, 2002·Annals of Clinical Biochemistry·David J Newman
Jul 10, 2003·Gastroenterology·Steven DooleyAxel M Gressner
Dec 16, 2003·The Journal of Biological Chemistry·Gabriela Velasco-LoydenFernando López-Casillas
Feb 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shu-Chen ChuHui-Ling Chiou

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Citations

Jul 16, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Lynda AoudjehaneFilomena Conti
Feb 9, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuekang XuJose A Villadangos
Sep 3, 2014·Biological Chemistry·Gilles LalmanachMariana Kasabova
Nov 2, 2013·Proteomics. Clinical Applications·Mariana KasabovaGilles Lalmanach
Aug 1, 2008·Molecular Biology of the Cell·Gunter MaubachLang Zhuo
Aug 30, 2016·World Journal of Gastroenterology : WJG·Norio KuboKen Shirabe
May 3, 2014·The Journal of Biological Chemistry·Mariana KasabovaGilles Lalmanach

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