Variable expression of human lactoferrin gene in mice milk driven by its 90 KB upstream flanking sequences

Animal Biotechnology
Zhaoliang LiuNing Li

Abstract

One major drawback in research of animal mammary gland bioreactors is the low production rate of high-expressing transgenic animals due to position effects. To obtain high and stable expression of foreign gene, yeast and bacterial artificial chromosome have been used as transgene vector in recent research. Human lactoferrin is a bioactive, versatile protein, and has large potential in nutritional and therapeutic applications. Therefore, production of recombinant lactoferrin using animal bioreactors was studied widely to satisfy its large requirement. We reported here a transgenic mice model with high-level expression of recombinant human lactoferrin in mammary gland. Transgene construct used here was a human bacterial artificial chromosome containing intact lactoferrin-encoding transcript unit, approximately 90 kb 5'-flanking sequences and 27.2 kb 3'-flanking sequences. We obtained totally 10 transgenic mice whereas two of them lacked of part of upstream sequences of the gene. Milk of eight transgenic mice line was detected by Western blot and radioimmunoassay and seven lines expressed recombinant human lactoferrin at high but variable level (0.29, 0.53, 0.90, 1.23, 2.76, 3.58, and 8.02 mg/mL, respectively). The variability of ...Continue Reading

References

Sep 1, 1991·Bio/technology·P KrimpenfortR Strijker
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R A McKnightL Hennighausen
Sep 11, 1990·Nucleic Acids Research·M W ReyF R Pieper
Jan 1, 1986·Annual Review of Genetics·R D Palmiter, R L Brinster
Mar 1, 1994·Transgenic Research·G J PlatenburgR Strijker
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D Siebert, B C Huang
Mar 26, 1999·Molecular Reproduction and Development·Y FujiwaraM Ueda
Jun 3, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·S BaveyeD Legrand
Jul 28, 1999·Molecular Reproduction and Development·Y FujiwaraM Ueda
Dec 22, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L H Vorland
Apr 18, 2001·Transgenic Research·P Giraldo, L Montoliu
May 1, 2002·Nature Biotechnology·Patrick H C van BerkelPeter H Nibbering

❮ Previous
Next ❯

Citations

Apr 20, 2006·Animal Biotechnology·Chunjiang ZhaoDinglian Zhang
Feb 15, 2014·Journal of Analytical Methods in Chemistry·Yu-Guo YuanYong Cheng

❮ Previous
Next ❯

Datasets Mentioned

BETA
U95626
AF508798

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

BLASTN
BioEdit

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.